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    蓝碧秀, 吴子恺, 王凛, 杨丽超, 阳丽艳, 何勇强, 姜伯乐. DGAT1-2基因在超高油玉米中的转化、表达及对油脂含量的影响[J]. 华南农业大学学报, 2015, 36(5): 43-47. DOI: 10.7671/j.issn.1001-411X.2015.05.008
    引用本文: 蓝碧秀, 吴子恺, 王凛, 杨丽超, 阳丽艳, 何勇强, 姜伯乐. DGAT1-2基因在超高油玉米中的转化、表达及对油脂含量的影响[J]. 华南农业大学学报, 2015, 36(5): 43-47. DOI: 10.7671/j.issn.1001-411X.2015.05.008
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    LAN Bixiu, WU Zikai, WANG Lin, YANG Lichao, YANG Liyan, HE Yongqiang, JIANG Bole. Transformation and expression of DGAT1-2 gene and its effect on the oil content of super-high oil corn[J]. Journal of South China Agricultural University, 2015, 36(5): 43-47. DOI: 10.7671/j.issn.1001-411X.2015.05.008
    Citation: LAN Bixiu, WU Zikai, WANG Lin, YANG Lichao, YANG Liyan, HE Yongqiang, JIANG Bole. Transformation and expression of DGAT1-2 gene and its effect on the oil content of super-high oil corn[J]. Journal of South China Agricultural University, 2015, 36(5): 43-47. DOI: 10.7671/j.issn.1001-411X.2015.05.008
    shu

    DGAT1-2基因在超高油玉米中的转化、表达及对油脂含量的影响

    Transformation and expression of DGAT1-2 gene and its effect on the oil content of super-high oil corn

      摘要
    • 摘要:
      目的 三酰甘油是油料作物油脂的主要成分, 二酰基甘油酰基转移酶(DGAT)是催化三酰甘油合成唯一的关键酶和限速酶.探讨DGAT基因对超高油玉米Zea mays L.种子油分含量的影响, 以进一步提高超高油玉米的含油率.
      方法 采用花粉管通道法将DGAT1-2基因导入超高油玉米自交系, 在盆栽条件下5~6叶期对T1代植株进行PCR检测筛选, Southern杂交进一步验证.成熟时收获10株自交授粉的转基因植株(T2代)籽粒, 测籽粒平均含油率, 通过t检验进行统计分析.
      结果和结论 初步筛选获得假定转化植株163株, Southern杂交获29株转基因植株, 平均转化率为1.44%. t检验结果表明, 10株自交授粉的转基因植株(T2)籽粒平均含油率比非转基因植株籽粒平均含油率提高了6.19%, 差异极显著(P < 0.01).利用花粉管通道法导入DGAT1-2基因, 可显著提高超高油玉米籽粒含油率, 是一种切实有效提高超高油玉米含油量的新途径.

       

      Abstract:
      Objective Triacylgycerol (TAG) is the main oil ingredient of oil-bearing crops. Diacylgycerol acyltransferase (DGAT) is the sole crucial rate-limiting enzyme in the synthesis process of triacylgycerol. The effect of DGAT gene on the oil content of super-high oil corn was investigated to improve the oil content of super-high oil corn.
      Method DGAT1-2 gene was transferred into super-high oil corn inbred lines by pollen-tube-pathway. Transgenic plants were obtained through screening with PCR among T1 generation potted plants at the 5-6-leaf stage. Transgenic plants were verified by Southern-blotting. The average oil content of transgenic T2 seeds was analyzed by the t-test.
      Result and conclusion One hundred and sixty-three putative transgenic plants were obtained through screening with PCR among a large number of T1 generation potted plants at the 5-6-leaf stage. Twenty-nine transgenic plants were verified by Southern-blotting. The average transformation rate was 1. 44%. The seeds of 10 transgenic T2 plants were harvested by self-pollination. The average oil content of transgenic T2 seeds was significantly higher than that of the non-transgenic plants by the t-test (P < 0. 01), with the increase rate being 6. 19%. The results provide a new practical and effective approach to improve the oil content of super high oil corn.

       

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