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    周均云, 习欠云, 关立增, 江青艳, 吴珍芳, 张永亮. 精子与慢病毒孵育制备转基因猪的分子检测及遗传分析[J]. 华南农业大学学报, 2015, 36(5): 7-11. DOI: 10.7671/j.issn.1001-411X.2015.05.002
    引用本文: 周均云, 习欠云, 关立增, 江青艳, 吴珍芳, 张永亮. 精子与慢病毒孵育制备转基因猪的分子检测及遗传分析[J]. 华南农业大学学报, 2015, 36(5): 7-11. DOI: 10.7671/j.issn.1001-411X.2015.05.002
    shu
    ZHOU Junyun, XI Qianyun, GUAN Lizeng, JIANG Qingyan, WU Zhenfang, ZHANG Yongliang. Molecular identification and inheritance analysis of transgenic pigs produced by incubation of sperm and lentivirus[J]. Journal of South China Agricultural University, 2015, 36(5): 7-11. DOI: 10.7671/j.issn.1001-411X.2015.05.002
    Citation: ZHOU Junyun, XI Qianyun, GUAN Lizeng, JIANG Qingyan, WU Zhenfang, ZHANG Yongliang. Molecular identification and inheritance analysis of transgenic pigs produced by incubation of sperm and lentivirus[J]. Journal of South China Agricultural University, 2015, 36(5): 7-11. DOI: 10.7671/j.issn.1001-411X.2015.05.002
    shu

    精子与慢病毒孵育制备转基因猪的分子检测及遗传分析

    Molecular identification and inheritance analysis of transgenic pigs produced by incubation of sperm and lentivirus

      摘要
    • 摘要:
      目的 在前期精子与慢病毒孵育法制备的转基因猪基础上，进一步探讨外源基因在转基因猪上的遗传状况.
      方法 通过转基因猪与野生猪交配扩繁后，运用PCR和Southern-blot法对转基因G1和G2代进行外源基因的检测与遗传分析.
      结果和结论 采用PCR法检测，G1代PCR阳性率为25.42%(15/59)；G2代PCR阳性率为46.67%(7/15).在12头阳性转基因猪的13种组织中，外源基因在多种组织中均有存在；对阳性转基因猪的胃、垂体、下丘脑组织的RT-PCR检测结果显示，外源基因在66.67%(24/36)的组织样品中均有表达.表明在精子与慢病毒共孵育法制备的转基因猪中，外源基因能整合到猪的基因组，并遗传给后代.

       

      Abstract:
      Objective This study investigated the inheritance analysis of foreign genes in transgenic pigs produced previously by using incubation of sperm and lentivirus.
      Method Transgenic pigs were reproduced by mating with wildtype pigs, and their offsprings of transgenic G1 and G2 were examined for inher-itance of foreign gene with PCR and Southern-blot methods.
      Result and conclusion The results showed that PCR-positive rate was 25.42%(15/59)and 46.67%(7/15)in G1 and G2 respectively.In the 13 tissues of the 12 positive transgenic pigs, the foreign genes were found in many tissues; RT-PCR detection showed 66.67%(24/36)tissue samples from stomach, pituitary and hypothalamus of positive pigs represented foreign gene expression.The results indicate foreign genes of G0 pigs produced by incubation of sperm and lentivirus can be integrated in the pig genome and transmitted to their offsprings.

       

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