Synthesis and biological activities of maganesium (Ⅱ) complex with 5-(chloromethyl) salicylaldehyde-tyrosine
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摘要:目的
在甲醇溶剂体系中合成5-氯甲基水杨醛缩L-酪氨酸Schiff碱配体及其Mn(Ⅱ)配合物.
方法通过元素分析、摩尔电导、红外光谱、紫外光谱及差热-热重方法确定它们的分子组成; 采用滤纸片法、光还原NBT法及荧光猝灭法测定该配合物的抗菌活性、SOD活性及其与牛血清白蛋白(BSA)的相互作用.
结果和结论5-氯甲基水杨醛缩L-酪氨酸Schiff碱配体及其Mn(Ⅱ)配合物的分子组成分别为K2L·3H2O(L = C17H14NO4Cl2-)和K[MnL(CH3COO)]·2H2O,配合物中Schiff碱配体上的亚胺基N、羧基O及酚羟基O均与Mn(Ⅱ)配位.该配合物的抗菌活性高于配体,且对革兰阴性菌大肠埃希菌的抗菌活性高于对革兰阳性菌金黄色葡萄球菌的; 该配合物具有较高的SOD活性,其IC50为1.616 μmol·L-1; 基于静态猝灭机理,该配合物能有效猝灭BSA的内源荧光,并与BSA结合形成1种基态复合物,25 ℃条件下与BSA的结合位点数n约为1,结合常数KA为1.62 × 106 L·mol-1,且分子内可能发生非辐射能量转移.
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关键词:
- Mn(Ⅱ)配合物 /
- 5-氯甲基水杨醛缩L-酪氨酸 /
- 抗菌活性 /
- SOD活性 /
- 牛血清白蛋白
Abstract:ObjectiveTo synthesize a novel Schiff base, 5-(chloromethyl) salicylaldehyde-tyrosine and its maganesium(Ⅱ) complex with methanol solvent.
MethodThe ligand and complex were characterized by elemental analysis, molar conductance, IR, UV and TG-DTA.Antibacterial activity, SOD activity of the complex and its interaction with bovine serum albumin (BSA) were examined by filter paper method, photoreduction of nitro-blue tetrazolium (NBT) and fluorescence quenching technique, respectively.
Result and conclusionThe ligand and complex formula were K2L·3H2O (L = C17H14NO4Cl2-) and K[MnL(CH3COO)]·2H2O, respectively.The nitrogen atom of imino group and the oxygen atoms of phenol group and carboxyl group in the ligand and one acetate ion were coordinated with the central metal Mn(Ⅱ). The antibacterial experiments in vitro showed that the complex had higher antibacterial activity than the Schiff base.The antibacterial activity against Escherichia coli as a gramnegative bacterium was higher than Staphylococcus aureus as a gram-positive bacterium. The complex had high SOD activity and its IC50 value was 1.616 μmol·L-1.The complex effectively quenches the intrinsic fluorescence of BSA via static quenching mechanism, and forming a ground-state complex with BSA. The number of binding sites (n) and the binding constant (KA) at 25 ℃ are about 1 and 1.62 × 106 L·mol-1 respectively, and non-radiation energy is transferred red from BSA to the complex possibly.
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