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    刘云华, 吴毅歆, 杨绍聪, 何鹏飞, 何月秋. 洋葱伯克霍尔德溶磷菌的筛选和溶磷培养条件优化[J]. 华南农业大学学报, 2015, 36(3): 78-82. DOI: 10.7671/j.issn.1001-411X.2015.03.014
    引用本文: 刘云华, 吴毅歆, 杨绍聪, 何鹏飞, 何月秋. 洋葱伯克霍尔德溶磷菌的筛选和溶磷培养条件优化[J]. 华南农业大学学报, 2015, 36(3): 78-82. DOI: 10.7671/j.issn.1001-411X.2015.03.014
    shu
    LIU Yunhua, WU Yixin, YANG Shaocong, HE Pengfei, HE Yueqiu. Screening of phosphorus-solubilizing strain Burkholderia cenocepacia and optimizing of phosphate-dissolving culture condition[J]. Journal of South China Agricultural University, 2015, 36(3): 78-82. DOI: 10.7671/j.issn.1001-411X.2015.03.014
    Citation: LIU Yunhua, WU Yixin, YANG Shaocong, HE Pengfei, HE Yueqiu. Screening of phosphorus-solubilizing strain Burkholderia cenocepacia and optimizing of phosphate-dissolving culture condition[J]. Journal of South China Agricultural University, 2015, 36(3): 78-82. DOI: 10.7671/j.issn.1001-411X.2015.03.014
    shu

    洋葱伯克霍尔德溶磷菌的筛选和溶磷培养条件优化

    Screening of phosphorus-solubilizing strain Burkholderia cenocepacia and optimizing of phosphate-dissolving culture condition

      摘要
    • 摘要:
      目的 从磷矿区植物根围土中，筛选出具有较高溶磷能力的菌株及优化溶磷培养条件.
      方法 通过稀释凃板法分离、筛选菌株，并进行全细胞脂肪酸分析和16S rDNA测序鉴定; 正交试验优化溶磷培养条件.
      结果和结论 筛选出22株具有溶磷能力的菌株，其中菌株YN2014102的溶磷能力最强，在10 g·L-1的磷矿粉培养基中能释放244.75 mg·L-1水溶性磷.经全细胞脂肪酸分析和16S rDNA测序，该菌株被鉴定为洋葱伯克霍尔德菌.正交试验的结果表明，在质量浓度10 g·L-1的磷矿粉、28 ℃、摇床转速为180 r·min-1的条件下培养5 d，溶磷效果最好，达277.08 mg·L-1.

       

      Abstract:
      Objective To isolate and screen phosphorus-solubilizing strains from the rhizospheric soil in phosphate ore zone and to optimize its phosphorus-solubilizing condition.
      Method Dilution and plating were applied to screen and purify phosphorus-solubilizing strains. The whole-cell fatty acid was analyzed by gas chromatography and 16S rDNA sequence was identified. The orthogonal design test was used in optimizing phosphorus-solubilizing condition.
      Result and conclusion Total 22 phosphorus-solubilizing strains were obtained by the traditional methodologies. YN2014102 strain showed a strong ability to dissolve phosphate, which could get 244.75 mg·L-1 available phosphorus from the 10 g·L-1 phosphate rock medium.YN2014102 strain was identified as Burkholderia cenocepacia based on its gas chromatography analysis of whole-cell fatty acid and 16S rDNA sequencing. When the bacterium was cultured in the 10 g·L-1 phosphate rock powder medium at 28 ℃ in a shaker with 180 r·min-1 for 5 days, the dissolve effect of solubilizing phosphorus was the best, which can obtain 277.08 mg·L-1 of available phosphorus in the medium.

       

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