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    张晖, 宋圆圆, 吕顺, 郭婧婧, 曾任森. 香蕉根际促生菌的抑菌活性及对作物生长的促进作用[J]. 华南农业大学学报, 2015, 36(3): 65-70. DOI: 10.7671/j.issn.1001-411X.2015.03.012
    引用本文: 张晖, 宋圆圆, 吕顺, 郭婧婧, 曾任森. 香蕉根际促生菌的抑菌活性及对作物生长的促进作用[J]. 华南农业大学学报, 2015, 36(3): 65-70. DOI: 10.7671/j.issn.1001-411X.2015.03.012
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    ZHANG Hui, SONG Yuanyuan, LÜ Shun, GUO Jingjing, ZENG Rensen. The antifungal activity and crop growth stimulation of growth-promoting rhizobacteria from banana rhizosphere soil[J]. Journal of South China Agricultural University, 2015, 36(3): 65-70. DOI: 10.7671/j.issn.1001-411X.2015.03.012
    Citation: ZHANG Hui, SONG Yuanyuan, LÜ Shun, GUO Jingjing, ZENG Rensen. The antifungal activity and crop growth stimulation of growth-promoting rhizobacteria from banana rhizosphere soil[J]. Journal of South China Agricultural University, 2015, 36(3): 65-70. DOI: 10.7671/j.issn.1001-411X.2015.03.012
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    香蕉根际促生菌的抑菌活性及对作物生长的促进作用

    The antifungal activity and crop growth stimulation of growth-promoting rhizobacteria from banana rhizosphere soil

      摘要
    • 摘要:
      目的 研究4种香蕉根际促生菌对香蕉枯萎病镰刀菌和几种重要作物病原菌的抑菌活性，及其对番茄和玉米的促生作用.
      方法 在培养皿中测定根际促生菌对作物病原菌的拮抗作用，通过盆栽试验测定根际促生菌发酵液对作物生长的促进作用.
      结果和结论 从健康香蕉根际土中分离获得的枯草芽孢杆菌Bacillus. subtilis、解淀粉芽孢杆菌B. amyloliquefaciens、耳炎假单胞菌Pseudomonas. otitidis和绿针假单胞菌Ps. choloeaphtis对香蕉枯萎病镰刀菌4号小种Fusarium oxysporum f. sp. cubense具有强烈的抑菌活性，对番茄早疫病菌Alternaria solani、玉米纹枯病菌Rhizoctonia solani、荔枝炭疽病菌Colletotrichum gloeosporioides Penz.、香蕉炭疽病菌C.musarum Cooke et Mass、青瓜枯萎病菌F.oxysporum (Schl.) f. sp cucumerinum Owen、小麦赤霉病菌F.graminearum和荔枝霜霉病菌Penorophythora litchi Chen等作物病原菌也具有很好的抑菌活性；4种香蕉根际土壤细菌中耳炎假单胞菌生长最快、抑菌效果最好，其生长半径分别是枯草芽孢杆菌、解淀粉芽孢杆菌、绿针假单胞菌的2.75、2.61和2.70倍，其对香蕉枯萎病、番茄早疫病菌、玉米纹枯病菌、荔枝炭疽病菌、香蕉炭疽病菌、青瓜枯萎病菌、小麦赤霉病菌和荔枝霜霉病的抑菌率分别达到50.70%、62.95%、70.85%、68.10%、58.58%、59.30%、51.34%和63.08%；用耳炎假单胞菌发酵液培养植物使番茄和玉米株高分别提高16%和33%，并且使番茄叶片总叶绿素含量增加40%.以上结果表明，香蕉根际微生物耳炎假单胞菌对作物病原菌有很好的抑菌活性，并且促进番茄和玉米的生长.

       

      Abstract:
      Objective To investigate the inhibitory effects of four banana growth-promoting rhizobacteria (PGPR) on Fusarium oxysporum f. sp. cubense race 4 and several other important crop pathogens.
      Method The antifungal activity of PGPR was evaluated in petri dish. The promoting effect of PGPR fermented broth on plant growth was determined in a pot experiment.
      Result and conclusion Four strains of PGPR including Bacillus subtilis, B. amyloliquefaciens, Pseudomonas otitidis and Ps. choloeaphtis isolated from rhizosphere soil of a healthy banana orchard showed a strong inhibitory effect on Fusarium oxysporum f. sp. cubense race 4, a causal agent of banana Fusarium wilt. The four bacteria also displayed antifungal activities against other tested crop pathogens including Alternaria solani, Rhizoctonia solani, Colletotrichum gloeosporioides Penz., C. musarum, F.oxysporum(Schl.) f.sp cucumerinum Owen, F. graminearum and Penorophythora litchi Chen. Ps. otitidis grew much faster than the other three bacteria, and the growth radius of Ps. otitidis was 2.75-, 2.61- and 2.70-fold relative to B. subtilis, B. amyloliquefaciens and Ps. choloeaphtis respectively. Ps. otitidis showed the best inhibitory effect on the tested crop pathogens, and the pathogen inhibition rate of Ps. otitidis to Fusarium oxysporum f. sp. cubense race 4, A. solani, R. solani, C. gloeosporioides Penz., C. musae, F.oxysporum (Schl.) f.sp cucumerinum Owen, F. graminearum and Pe. litchi was 50.70%, 62.95%, 70.85%, 68.10%, 58.58%, 59.30%, 51.34% and 63.08% respectively. The fermented broth of Ps. otitidis stimulated the seedling growth of tomatoes and corns, and it increased their shoot height by 16% and 33% respectively. It also increased the chlorophyll content of tomatoes by 40%. These results suggest that Ps. otitidis has strong antifungal activity against crop pathogens and also stimulate the growth of tomatoes and corns.

       

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