Objective This experiment was conducted to investigate the resistance genes in bacteria from vegetable samples adjacent to the swine farms in Southern China.

Method PCR and sequencing were performed to detect the resistance genes of aminoglycoside, tetracycline, beta lactamase, quinolone, macrolide and clindamycin.

Result and conclusion Tetracycline resistance genes had a wide distribution range (the ground detectable rate of this kind of gene was 90% (9/10) both in accessory bacteria and endophytic bacteria).There were different resistance genes between accessory bacteria and endophytic bacteria from vegetables, and accessory bacteria had richer resistance genes than endophytic bacteria. The detectable rates of resistance genes in accessory bacteria of A, B, C, D, E, F, G, H, I and J samples were 77.78% (21/27), 92.59% (25/27), 92.59% (25/27), 51.85% (14/27), 33.33% (9/27), 92.59% (25/27), 77.78% (21/27), 7.41% (2/27), 81.48% (22/27) and 77.78% (21/27), respectively; the detectable rates of resistance genes in endophytic bacteria were 0 (0/27), 11.11% (3/27), 11.11% (3/27), 11.11% (3/27), 29.63% (8/27), 48.15% (13/27), 29.63% (8/27), 33.33% (9/27), 25.93% (7/27) and 22.22% (6/27), respectively.The results indicate that the resistance problem in bacteria, especially accessory bacteria from vegetables, is probably very serious.It can even inhibit the growth of crops and threaten animal and human health through food chain to which enough importance should be attached.