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    施琼, 胡峰, 黄烈健, 陈应彪. 马大杂种相思组培快繁技术[J]. 华南农业大学学报, 2015, 36(2): 79-84. DOI: 10.7671/j.issn.1001-411X.2015.02.014
    引用本文: 施琼, 胡峰, 黄烈健, 陈应彪. 马大杂种相思组培快繁技术[J]. 华南农业大学学报, 2015, 36(2): 79-84. DOI: 10.7671/j.issn.1001-411X.2015.02.014
    shu
    SHI Qiong, HU Feng, HUANG Liejian, CHEN Yingbiao. In vitro propagation of Acacia mangium×A. auriculiformis[J]. Journal of South China Agricultural University, 2015, 36(2): 79-84. DOI: 10.7671/j.issn.1001-411X.2015.02.014
    Citation: SHI Qiong, HU Feng, HUANG Liejian, CHEN Yingbiao. In vitro propagation of Acacia mangium×A. auriculiformis[J]. Journal of South China Agricultural University, 2015, 36(2): 79-84. DOI: 10.7671/j.issn.1001-411X.2015.02.014
    shu

    马大杂种相思组培快繁技术

    In vitro propagation of Acacia mangium×A. auriculiformis

      摘要
    • 摘要:
      目的 提高马大杂种相思Acacia mangium×A. auriculiformis良种的快速繁殖效率与推广种植力度.
      方法 以马大杂种相思新生枝条带腋芽茎段为外植体，研究马大杂种相思组培快繁体系.
      结果和结论 用质量分数为0.1%的升汞和体积分数为75%的乙醇分别处理当年新生枝条第3~5个腋芽茎段15 min和30 s，然后接种至MS培养基进行初代培养，芽诱导率为95.68%；最佳增殖培养基为改良MS+6-BA 1.5 mg·L-1+NAA 0.1 mg·L-1+蔗糖30 ~40 g·L-1，35 d的有效增殖倍数为3.97；将增殖芽接入含IBA 600 mg·L-1的MS固体培养基上预培养4~8 h后转接至无激素1/2 MS培养基上，15 d即可全部生根；或将增殖芽直接接入1/2 MS+IBA 1.0 mg·L-1+NAA 0.5 mg·L-1+蔗糖30 g·L-1生根培养基上，第15天时生根率为99.43%.将生根苗移栽至以黄心土为基质的营养袋内，存活率为94.67%.

       

      Abstract:
      Objective To improve the propagation efficiency and extend the planting of Acacia mangium×A. auriculiformis.
      Method This study was carried out to explore the techniques of in vitro propagation of A.mangium×A. auriculiformis using stem segments with buds collected from 16-year plants as the explant.
      Result and conclusion Current season stem segments carrying 3-5 axillary buds were sterilized with w=0.1% mercuric chloride and φ＝75% alcoho1 for 15 min and 30 s respectively before they were inoculated onto MS medium. Buds could be induced successfully on MS with a shooting rate of 95.68%. The bud proliferation index was 3.97 after being transferred onto MS+6-BA 1.5 mg·L-1+NAA 0.1 mg·L-1+ sucrose 30-40 g·L-1 for 35 d. The buds all rooted on the 15th day from inoculation onto 1/2 MS medium after pre-cultured on MS supplemented with IBA 600 mg·L-1 for 4-8 h. Inoculating the proliferated buds onto 1/2 MS+IBA 1.0 mg·L-1+NAA 0.5 mg·L-1+sucrose 30 g·L-1 also generated a high rooting rate of 99.43%. The survival rate reach 94.67% after the rooted plantlet are transplanted to the yellow soil medium.

       

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