Screening and comprehensive evaluation of drought resistance indices of rice at germination stage
-
摘要:目的
探索干旱胁迫对水稻萌芽期生长的影响，筛选水稻萌芽期抗旱性指标.
方法以粳型、籼型常规稻及空间诱变稳定后代株系等共62个水稻品种(系)为试验材料，利用聚乙二醇(PEG-6000)模拟干旱胁迫，研究了干旱处理对水稻萌发期芽鞘长、最长根长、芽干质量、根冠比等8个形态和生理指标的变化.利用隶属函数分析方法对所有材料进行综合抗旱能力评价.
结果和结论干旱胁迫严重影响了水稻的萌发，对根长的抑制最明显；根干物质积累比芽干物质积累受干旱胁迫的抑制更明显.芽鞘长和最长根长与水稻萌芽期综合抗旱能力极显著相关，且2个指标间无相关性，适合用作水稻萌发期抗旱鉴定指标.根据水稻萌发期抗旱性和综合抗旱能力评价，从62个水稻品种(系)中筛选出14个高抗旱品种(系)，用于后续抗旱研究.
Abstract:ObjectiveTo study the effect of drought stress on rice growth at germination stage and screen rice drought resistance indicators quickly and efficiently.
MethodSixty-two rice varieties (lines), including indicia and japonica rice and space-induced mutants, were studied under drought artificially created by PEG-6000. Eight indicators such as shoot length, maximum root length, coleoptile length, root number and shoot dry mass etc. were tested under stressed and control conditions.
Result and conclusionRice germination, especially the root length, was seriously affected by drought stress, and the impact of drought stress on root dry matter accumulation was much greater than that of shoot dry matter accumulation. Two indicators of maximum root length and coleoptile length significantly correlated with the comprehensive drought capability which were suitable for drought resistance screening at rice germination stage. Fourteen out of 62 materials have been identified to be highly drought resistant through dynamic clustering statistics, which will be promising for rice drought resistance breeding.
-
Keywords:
- rice /
- germination stage /
- drought stress /
- PEG /
- drought resistance /
- screening index
-
-
![]()
图 1 部分水稻品系的种子萌发后第8天的生长状况
Figure 1. Seed germination status of some rice varieties in the 8th day
表 1 水稻萌芽期抗干旱指标间的相关系数1)
Table 1 Related coefficients of drought resistance indicators at rice germination stage
下载: 导出CSV
表 2 水稻萌芽期抗旱性综合指标CI(x)特征值和特征向量
Table 2 Characteristic values and vectors of integrated indicators of drought resistance at rice germination stage
下载: 导出CSV
表 3 水稻萌芽期各品种(系)D值及抗旱性综合评价1)
Table 3 The D values and comprehensive appraisal of drought resistance of rice varieties(strains) during germination stage
下载: 导出CSV
-
[1] MIURA K, IKEDA M, MATSUBARA A, et al.OsSPL14 promotes panicle branching and higher grain productivity in rice[J]. Nat Genet, 2010, 42:545-549. doi: 10.1038/ng.592
[2] LUO L. Breeding for water-saving and drought-resistance rice (WDR) in China[J]. J Exp Bot, 2010, 61 (13): 3509-3517. doi: 10.1093/jxb/erq185
[3] ZHANG Q. Strategies for developing green super rice[J]. Proc Natl Acad Sci USA, 2007, 104(42): 16402-16409. doi: 10.1073/pnas.0708013104
[4] CATTIVELLI L, RIZZA F, BADECK F, et al. Drought tolerance improvement in crop plants: An integrated view from breeding to genomics[J]. Field Crops Res, 2008, 105(1): 1-14. http://cn.bing.com/academic/profile?id=5e21fcc5e16e261a8ddd5aa7e1c241c5&encoded=0&v=paper_preview&mkt=zh-cn
[5] ZHANG J, JIA W, YANG J, et al. Role of ABA in integrating plant responses to drought and salt stresses[J]. Field Crops Res, 2006, 97(1): 111-119. doi: 10.1016/j.fcr.2005.08.018
[6] 王贺正, 马均, 李旭毅, 等.水稻开花期抗旱性鉴定指标的筛选[J].作物学报, 2005, 31(11):1485-1489. doi: 10.3321/j.issn:0496-3490.2005.11.016 [7] 胡标林, 李名迪, 万勇, 等.东乡普通野生稻全生育期抗旱性鉴定[J].作物学报, 2007, 33(3):425-432. http://d.old.wanfangdata.com.cn/Periodical/zuowxb200703012 [8] 戴高兴, 彭克勤, 邓国富, 等.聚乙二醇模拟干旱对耐低钾水稻幼苗的根系生长及某些生理特性的影响[J].植物生理学通讯, 2007, 43(5):865-868. http://d.old.wanfangdata.com.cn/Periodical/zwslxtx200705014 [9] CAPELL T, BASSIE L, CHRISTOU P. Modulation of the polyamine biosynthetic pathway in transgenic rice confers tolerance to drought stress[J]. Proc Natl Acad Sci USA, 2004, 101(26):9909-9914. doi: 10.1073/pnas.0306974101
[10] KATO Y, HIROTSU S, NEMOTO K, et al. Identification of QTLs controlling rice drought tolerance at seedling stage in hydroponic culture[J]. Euphytica, 2008, 160(3):423-430. doi: 10.1007/s10681-007-9605-1
[11] 麦苗苗, 石大兴, 王米力, 等.PEG处理对连香树种子萌发与芽苗生长的影响[J].林业科学, 2009, 45(10):94-99. http://d.old.wanfangdata.com.cn/Periodical/lykx200910016 [12] BLUM A. An evaluation of seed and seedling drought tolerance screening tests in wheat[J].Euphytica, 1980, 29: 727-736. doi: 10.1007/BF00023219
[13] 薛慧勤, 甘信民, 顾淑媛, 等.花生种子萌发特性和抗旱性关系的高渗溶液法[J].中国油料作物学报, 1997, 19(3):30-33. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=CAS201303040000354130 [14] 王玉萍, 刘庆昌, 李爱贤, 等.甘薯耐旱突变体的离体筛选与鉴定[J].中国农业科学, 2003, 36(9):1000-1005. doi: 10.3321/j.issn:0578-1752.2003.09.004 [15] 龚明.作物抗旱性鉴定方法与指标及其综合评价[J].云南农业大学学报, 1989, 4(1):73-84. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=QK000003584452 [16] 童继平, 吴跃进, 郑乐娅, 等.水稻超高产育种若干问题的评述与讨论[J].种子, 2003(2):60-64. doi: 10.3969/j.issn.1001-4705.2003.02.025 [17] 原小燕, 符明联, 何晓莹, 等.不同抗旱性油菜种子萌发期抗旱指标比较研究[J].干旱地区农业研究, 2012, 30(5):77-81. doi: 10.3969/j.issn.1000-7601.2012.05.015 [18] 王玮, 邹琦, 杨兴洪, 等.水分胁迫下冬小麦芽鞘长度与抗旱性的关系及其遗传特性的研究[J].西北植物学报, 1997, 17(4):495-498. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=QK199800052676 [19] 胡颂平, 杨华, 邹桂花, 等.水稻胚芽鞘长度与抗旱性的关系及QTL定位[J].中国水稻科学, 2006, 20(1):19-24. doi: 10.3321/j.issn:1001-7216.2006.01.004 [20] 王贺正, 马均, 李旭毅, 等.水稻种质芽期抗旱性和抗旱性鉴定指标的筛选研究[J].西南农业学报, 2004, 17(5):594-599. doi: 10.3969/j.issn.1001-4829.2004.05.011
计量
- 文章访问数: 3452
- HTML全文浏览量: 34
- PDF下载量: 1480
