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    旷代, 许学斌, 张建民, 杨筱薇, 石维敏, 陶燕, 潘海建, 罗开健, 任涛. 上海市动物源食品中山夫登堡沙门菌耐药性及脉冲场凝胶电泳分型研究[J]. 华南农业大学学报, 2015, 36(1): 113-116. DOI: 10.7671/j.issn.1001-411X.2015.01.021
    引用本文: 旷代, 许学斌, 张建民, 杨筱薇, 石维敏, 陶燕, 潘海建, 罗开健, 任涛. 上海市动物源食品中山夫登堡沙门菌耐药性及脉冲场凝胶电泳分型研究[J]. 华南农业大学学报, 2015, 36(1): 113-116. DOI: 10.7671/j.issn.1001-411X.2015.01.021
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    KUANG Dai, XU Xuebin, ZHANG Jianmin, YANG Xiaowei, SHI Weimin, TAO Yan, PAN Haijian, LUO Kaijian, REN Tao. Antimicrobial resistance and pulse field gel electrophoresis analyses of Salmonella Senftenberg from food of animal origins in Shanghai, China[J]. Journal of South China Agricultural University, 2015, 36(1): 113-116. DOI: 10.7671/j.issn.1001-411X.2015.01.021
    Citation: KUANG Dai, XU Xuebin, ZHANG Jianmin, YANG Xiaowei, SHI Weimin, TAO Yan, PAN Haijian, LUO Kaijian, REN Tao. Antimicrobial resistance and pulse field gel electrophoresis analyses of Salmonella Senftenberg from food of animal origins in Shanghai, China[J]. Journal of South China Agricultural University, 2015, 36(1): 113-116. DOI: 10.7671/j.issn.1001-411X.2015.01.021
    shu

    上海市动物源食品中山夫登堡沙门菌耐药性及脉冲场凝胶电泳分型研究

    Antimicrobial resistance and pulse field gel electrophoresis analyses of Salmonella Senftenberg from food of animal origins in Shanghai, China

      摘要
    • 摘要:
      目的 了解上海市动物源食品中山夫登堡沙门菌Salmonella Senftenberg的流行情况、耐药性和分子分型情况.
      方法 于2008—2012年在上海分离鉴定得到15株动物食品源山夫登堡沙门菌，通过琼脂稀释法进行最小抑菌浓度测定，并应用脉冲场凝胶电泳技术(PFGE)对菌株进行分子分型研究.
      结果和结论 15株山夫登堡沙门菌有7株对磺胺异恶唑具有耐药性(46.7%)，除1株对链霉素耐药外(6.67%)，对其他抗生素均较为敏感.PFGE分型分为10个基因型.部分不同来源的基因型(X3，X4，X5)具有高度的相似性(88.2%)，表明这些不同来源的菌株可能存在流行相关性.

       

      Abstract:
      Objective To investigate the antimicrobial resistance and molecular types of Salmonella Senftenberg isolates from food of animal origins in Shanghai.
      Method A total of 15 Salmonella Senftenberg isolates were collected from 2008 to 2012 in Shanghai. Agar dilution method and pulse field gel electrophoresis (PFGE) were applied to the determination of minimum inhibitory concentrations (MICs) and molecular typing, respectively.
      Result and conclusion The 15 isolates exhibited resistance most often to sulfisoxazole(46.7%)and they were fully sensitive to the remaining antibiotics expect for one which was resistant to streptomycin (6.67%). PFGE analyses of 15 Salmonella Senftenberg isolates resulted in 10 unique patterns, among which three (X3, X4, X5) were grouped together at a high similarity index of 88.2%, su-ggesting that these isolates of different origins may be relevant in the epidemiology.

       

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