Abstract: 【Objective】 To expand biocontrol strain resources and screen new strains with a higher antagonistic rate and a wider inhibiting range to control ginseng diseases.【Method】 The antagonistic strains were isolated by dilution plate method and were screened by flat-stand method.【Result and conclusion】 Seven actinomycetes strains with higher antagonistic activities to single ginseng pathogen, such asPhytophthora cactorum, Sclerotinia schinseng, Botrytis cinerea, Alternaria panax, Cylindrocarpon destructans, Rhizoctonia solani and Fusarium solani, had been screened, whose inhibition rates were 80.84%, 82.46%, 66.08%, 71.33%, 64.63%, 85.53% and 65.11%, respectively. Five strains, including a strain of Trichoderma and four strains of Actinomyces, had higher antagonistic activities to seven kinds of ginseng pathogens. The results indicate that the beneficial strains in the rhizosphere has a biocontrol potential ability of ginseng pathogens and can be used to prevent and cure major diseases of ginseng.