陈燕华, 黄大辉, 邱永福, 张月雄, 刘芳, 马增凤, 刘驰, 李容柏. 水稻主要农艺性状的 QTL 分析[J]. 华南农业大学学报, 2014, 35(5): 42-51. DOI: 10.7671/j.issn.1001-411X.2014.05.008
    引用本文: 陈燕华, 黄大辉, 邱永福, 张月雄, 刘芳, 马增凤, 刘驰, 李容柏. 水稻主要农艺性状的 QTL 分析[J]. 华南农业大学学报, 2014, 35(5): 42-51. DOI: 10.7671/j.issn.1001-411X.2014.05.008
    CHEN Yanhua, HUANG Dahui, QUI Yongfu, ZHANG Yuexiong, LIU Fang, MA Zengfeng, LIU Chi, LI Rongbai. A QTL analysis of main agronomic characters in rice,Oryza sativa[J]. Journal of South China Agricultural University, 2014, 35(5): 42-51. DOI: 10.7671/j.issn.1001-411X.2014.05.008
    Citation: CHEN Yanhua, HUANG Dahui, QUI Yongfu, ZHANG Yuexiong, LIU Fang, MA Zengfeng, LIU Chi, LI Rongbai. A QTL analysis of main agronomic characters in rice,Oryza sativa[J]. Journal of South China Agricultural University, 2014, 35(5): 42-51. DOI: 10.7671/j.issn.1001-411X.2014.05.008

    水稻主要农艺性状的 QTL 分析

    A QTL analysis of main agronomic characters in rice,Oryza sativa

    • 摘要: 【目的】通过对水稻 Oryza sativa 主要农艺性状的遗传研究,挖掘优异主效QTL,为利用分子标记辅助选择进行高产、优质育种提供理论基础.【方法】以粳稻品系中野1211与籼稻品系中大304构建的重组自交系群体188个家系为试验材料,利用该重组自交系群体构建的含有142个SSR标记分子连锁图,采用区间作图法对抽穗期、单株有效穗数、株高、穗长、每穗实粒数、每穗总粒数、结实率、穗着粒密度、粒长、粒宽、粒形、千粒质量和单株质量13个主要农艺性状进行早、晚季的全基因组QTL定位.【结果和结论】除株高没有检测到相关的QTL之外,其余的每个性状检测到的QTL数目为2~15个.12个性状共检测到73个QTL,分布于水稻的12条染色体上,其中仅在早季能检测到的有34个,仅在晚季能检测到的有23个,两季都能检测到的只有16个.单个QTL的贡献率在5.3%~28.4%之间,其中超过20%以上的有10个.检测到的新位点为12个.早晚两季在多数染色体的多处区段上均检测到多个紧密连锁的QTL.检测到的12个新位点为水稻主要农艺性状的QTL定位增加了新的遗传信息,重复检测到的16个QTL可用于水稻分子标记辅助育种.

       

      Abstract: 【Objective】 To identify excellent main effect QTLs conferring high yield, high quality in rice.【Method】 A genetic linkage map consisting of 142 simple sequence repeat (SSR) markers was constructed based on a recombinant inbred line (RIL) population derived from a cross between Japonica Zhongye 1211 and Indica Zhongda 304 in rice,Oryza sativa. Through an interval mapping method, a genome-wide quantitative trait loci (QTL) analysis was carried out for 13 agronomic traits of rice in the early and late seasons in 2012, including heading period, effective tillers per plant, plant height, panicle length, filled grains per plant, grains per panicle, seed setting rate, grain density per panicle, grain length, grain width, grain shape, 1000-grain mass, grain mass per plant.【Result and conclusion】 The number of QTLs related with the traits except that plant height was 2-15. A total of 73 QTLs for 12 traits were identified and mapped on all of the 12 rice chromosomes. Among all the 73 QTLs, there were 34 QTLs identified in the early season rice, 23 QTLs in the late season rice, and 16 QTLs in both of the early and late season rices. Each of the QTLs individually explained 5.3%-28.4% of the phenotypic variations, among which 10 QTLs were more than 20%. In both cropping seasons, many QTLs closely linked each other were detected in multiple sections of most chromosomes. Twelve new QTL loci are discovered, and 16 QTLs are identified in both cropping seasons can be used in marker-assistant-selected method.

       

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