Abstract: 【Objective】A method for rapid determination of five fluoroquinolones (norfloxacin，ciprofloxacin，danofloxacin，enrofloxacin，sarafloxacin) residues in chicken muscle using high performance liquid chromatography (HPLC) was established．【Method】The extracting solution of the chicken samples included a mixture of acetonitrile and trichloroacetic acid(the mass fraction of 2%). HPLC mobile phase consisted of acetonitrile and phosphate buffer (0.02 mol·L^-1) with triethy lamine. Fluorometric excitation wavelength was 280 nm, and emission wavelength was 450 nm. 【Result and conclusion】The recoveries of 5 fluoroquinolones were 84.71%-99.66% at three spiked level(low, medium, high), and the relative standard deviations (RSD) were 0.20%-4.34%.The limit of quantiation (LOQ) of norfloxacin, ciprofloxacin and enrofloxacin was 10 μg·kg^-1. The LOQ of danofloxacin was 2 μg·kg^-1， and the sarafloxacin was 20 μg·kg^-1. The method is simple, practical, and sensitive. It can meet the needs of determination of fluoroquinolones in chicken muscle.