初志战, 巫光宏, 刘小林. 玉米醇溶蛋白的提取及酶解工艺研究[J]. 华南农业大学学报, 2014, 35(3): 86-89. DOI: 10.7671/j.issn.1001-411X.2014.03.016
    引用本文: 初志战, 巫光宏, 刘小林. 玉米醇溶蛋白的提取及酶解工艺研究[J]. 华南农业大学学报, 2014, 35(3): 86-89. DOI: 10.7671/j.issn.1001-411X.2014.03.016
    CHU Zhizhan, WU Guanghong, LIU Xiaolin. A research on extraction and enzymatic hydrolysis of zein[J]. Journal of South China Agricultural University, 2014, 35(3): 86-89. DOI: 10.7671/j.issn.1001-411X.2014.03.016
    Citation: CHU Zhizhan, WU Guanghong, LIU Xiaolin. A research on extraction and enzymatic hydrolysis of zein[J]. Journal of South China Agricultural University, 2014, 35(3): 86-89. DOI: 10.7671/j.issn.1001-411X.2014.03.016

    玉米醇溶蛋白的提取及酶解工艺研究

    A research on extraction and enzymatic hydrolysis of zein

    • 摘要: 【目的】优化水解醇溶蛋白生产功能肽的工艺,提高玉米醇溶蛋白的应用价值.【方法】通过正交试验获得从玉米蛋白粉中提取醇溶蛋白的最佳提取时间、提取温度、料液比、乙醇浓度等提取条件;通过碱性蛋白酶和蛋白酶 K 对玉米醇溶蛋白进行水解生产玉米多肽.【结果和结论】通过正交试验获得了从玉米蛋白粉中提取醇溶蛋白的最佳条件,即:料液比1∶4(g/mL),60 ℃水浴加热,乙醇体积分数为 75%,提取 3 h.两步酶解法效率高于单酶解法,两步酶解法先加蛋白酶 K 反应后再加碱性蛋白酶时水解效果最佳.

       

      Abstract: 【Objective】The production of short peptide from zein was investigated to greatly promote the application of zein.【Method】The conditions such as extraction time, temperature, ethanol concentration and ratio of liquid to solid were optimized by orthogonal test. Zein was hydrolyzed by protease K and alkaline protease in this study.【Result and conclusion】The optimal conditions of extracting zein were as follows: the ratio of solid to liquid was 1∶4; the extracting time was 3 hours; the extracting temperature was 60 ℃ and the concentration of ethanol was 75%. Results showed that the zein could be hydrolyzed more efficiently by combining two enzymes than by only using one of the two enzymes. Zein hydrolyzed by protease K and alkaline protease in turn is the best enzymatic hydrolysis method.

       

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