高效液相色谱 - 串联质谱法研究吡唑醚菌酯在人参根、茎、叶和土壤中的残留动态及最终残留量

王燕; 王春伟; 高洁; 许允成; 崔丽丽

doi:10.7671/j.issn.1001-411X.2014.03.013

高效液相色谱 - 串联质谱法研究吡唑醚菌酯在人参根、茎、叶和土壤中的残留动态及最终残留量

Determination of residual dynamics and final residues of pyraclostrobin in the ginseng root, stem, leaf and soil by HPLC-MS/MS

摘要

摘要: 【目的】明确在人参生长期施用250 g·L^-1吡唑醚菌酯乳油在人参根、茎、叶和土壤中的残留动态及最终残留量.【方法】样品经丙酮提取，N-丙基乙二胺(PSA)固相萃取柱净化后，用液相色谱-串联质谱法检测.【结果和结论】施药剂量为666.67 g·hm^-2(以有效成分计)时，吡唑醚菌酯在人参根、茎、叶和土壤中的降解半衰期为6.35~8.75 d.施药剂量为333.33~666.67 g·hm^-2时，施药后60 d吡唑醚菌酯在人参根、茎、叶和土壤中的最终残留量低于0.020 6 mg·kg^-1，因此建议施用250 g·L^-1吡唑醚菌酯乳油时，施药剂量不高于666.67 g·hm^-2，施药1 次，安全间隔期为35 d.

Abstract: 【Objective】To identify the residual dynamics and final residues of pyraclostrobin 250 g·L^-1 EC in the ginseng root, stem, leaf and soil after spraying during the ginseng growth period.【Method】The samples were extracted with acetone, cleaned up with primary secondary amine (PSA) solid phase extraction cartridge, and determined by high performance liquid chromatography coupled with electrospray mass spectrometry (HPLC-MS/MS).【Result and conclusion】The half lives of pyraclostrobin were from 6.35 to 8.75 d in the ginseng root, stem, leaf and soil with a spraying dosage of 666.67 g·hm^-2. Final residues of pyraclostrobin in the ginseng root, stem, leaf and soil were below 0.020 6 mg·kg^-1 over 60 d at a spraying dosage ranging from 333.33 to 666.67 g·hm^-2. It is recommended that the preharvest interval of pyraclostrobin 250 g·L^-1 EC should be set 35 d and sprayed one time at the dosage of no more than 666.67 g·hm^-2.

HTML全文

参考文献(0)

施引文献

资源附件(0)