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    康帅, 殷中琼, 贾仁勇, 代如意, 李莉, 彭练慈, 曲径, 梁陈. 乌梅等20种中药对胸膜肺炎放线杆菌的体外抗菌活性研究[J]. 华南农业大学学报, 2014, 35(3): 13-17. DOI: 10.7671/j.issn.1001-411X.2014.03.003
    引用本文: 康帅, 殷中琼, 贾仁勇, 代如意, 李莉, 彭练慈, 曲径, 梁陈. 乌梅等20种中药对胸膜肺炎放线杆菌的体外抗菌活性研究[J]. 华南农业大学学报, 2014, 35(3): 13-17. DOI: 10.7671/j.issn.1001-411X.2014.03.003
    shu
    KANG Shuai, YIN Zhongqiong, JIA Renyong, DAI Ruyi, LI Li, PENG Lianci, QU Jing, LIANG Chen. A study on antibacterial activity of crude extract from twenty traditional Chinese medicines like Fructus mume against Actinobacillus pleuropneumoniae in vitro[J]. Journal of South China Agricultural University, 2014, 35(3): 13-17. DOI: 10.7671/j.issn.1001-411X.2014.03.003
    Citation: KANG Shuai, YIN Zhongqiong, JIA Renyong, DAI Ruyi, LI Li, PENG Lianci, QU Jing, LIANG Chen. A study on antibacterial activity of crude extract from twenty traditional Chinese medicines like Fructus mume against Actinobacillus pleuropneumoniae in vitro[J]. Journal of South China Agricultural University, 2014, 35(3): 13-17. DOI: 10.7671/j.issn.1001-411X.2014.03.003
    shu

    乌梅等20种中药对胸膜肺炎放线杆菌的体外抗菌活性研究

    A study on antibacterial activity of crude extract from twenty traditional Chinese medicines like Fructus mume against Actinobacillus pleuropneumoniae in vitro

      摘要
    • 摘要: 【目的】观察乌梅 Fructus mume 等20种中药的体外抗胸膜肺炎放线杆菌 Actinobacillus pleuropneumoniae活性.【方法】 通过乙醇回流、水煎煮和超声波等方法对20种中药进行提取，采用二倍稀释法测定其对胸膜肺炎放线杆菌的体外抗菌活性；并研究抗菌活性较强的几味中药的体外联合抑菌活性.【结果和结论】 乌梅、黄连 Rhizoma coptidis、诃子 Terminalia chebula、秦皮 Cortex fraxini、地榆 Sanguisorbae officinalis、虎杖 Polygonum cuspidatum 6种中药提取物对胸膜肺炎放线杆菌的最小抑菌浓度 (Minimal inhibitory concentration，MIC)范围是6.25~50.00 mg/mL；大青叶 Isatis indigotica、茵陈 Artemisia capillaris、甘草 Glycyrrhiza uralensis 和白鲜皮 Dictamnus dasycarpus 4种中药提取物的MIC范围是50.00~100.00 mg/mL；黄柏 Phellodendron amurense、杜仲 Eucommia ulmoides、金银花 Lonicera japonica、柴胡 Bupleurum chinense、蒲公英 Taraxacum mongolicum、板蓝根 Baphicacanthus cusia、栀子 Gardenia jasminoides 和黄芪 Astragalus membranaceus 等10种中药提取物的 MIC>100.00 mg/mL.联合抑菌试验结果表明，乌梅、黄连、诃子和虎杖两两联合抑菌指数（FICI）≤1，乌梅、虎杖分别与秦皮的 FICI＞2.乌梅、黄连、诃子、虎杖、秦皮、地榆对胸膜肺炎放线杆菌均具有较好的体外抗菌活性；乌梅、黄连、诃子和虎杖两两联合为相加、协同作用；秦皮分别与乌梅、虎杖为拮抗作用.

       

      Abstract: 【Objective】The antibacterial activity of twenty traditional Chinese medicines against Actinobacillus pleuropneumoniae in vitro was evaluated in this study. 【Method】Twenty traditional Chinese medicines like Fructus mume were extracted by ethanol reflux extraction,water decoction and ultrasonic wave. Minimal inhibitory concentrations(MICs) of twenty traditional Chinese medicines against A. pleuropneumoniae were determined by the macrodilution broth method. The joint antimicrobial activity of some traditional Chinese medicines was evaluated in vitro.【Result and conclusion】The results showed that the MICs of the extract obtained from F. mume, Rhizoma coptidis, Terminalia chebula, Cortex fraxini, Sanguisorbae officinalis and Polygonum cuspidatum against A. pleuropneumoniae ranged from 6.25 to 50.00 mg/mL. MICs of the extract obtained from Isatis indigotica, Artemisia capillaris, Glycyrrhiza uralensis and Dictamnus dasycarpus ranged from 50.00 to 100.00 mg/mL. MICs of the extract obtained from Phellodendron amurense, Eucommia ulmoides, Lonicera japonica, Bupleurum chinense, Taraxacum mongolicum, Baphicacanthus cusia, Gardenia jasminoides and Astragalus membranaceus were higher than 100.00 mg/mL. The results of joint bacteriostatic test showed that the fractional inhibitory concentration index (FICI) of the pairwise combination of F. mume, R. coptidis, Te. chebula and Po. cuspidatum was lower than or equal to 1, and FICI of C. fraxini with Po. cuspidatum and F. mume was higher than 2. The F. mume, R. coptidis, Te. chebula, Po. cuspidatum, C. fraxini and S. officinalis had a good antimicrobial activity. The pairwise combination of F. mume, R. coptidis, Te. chebula and Po. cuspidatum showed an enhanced or additive synergy action, and C. fraxini showed antagonistic action with F. mume and Po. cuspidatum, respectively.

       

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