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    王 璐, 段冉冉, 赵 盼, 周晓华. 含 2 - 氨甲基苯并咪唑铜(Ⅱ)配合物的合成、表征、抑菌活性及与 DNA 作用的研究[J]. 华南农业大学学报, 2014, 35(2): 90-95. DOI: 10.7671/j.issn.1001-411X.2014.02.017
    引用本文: 王 璐, 段冉冉, 赵 盼, 周晓华. 含 2 - 氨甲基苯并咪唑铜(Ⅱ)配合物的合成、表征、抑菌活性及与 DNA 作用的研究[J]. 华南农业大学学报, 2014, 35(2): 90-95. DOI: 10.7671/j.issn.1001-411X.2014.02.017
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    WANG Lu, DUAN Ranran, ZHAO Pan, ZHOU Xiaohua. Syntheses，characteristics，bacteriostatic activities and their interaction with DNA of copper(Ⅱ) complexes containing 2-aminomethylbenzimidazole[J]. Journal of South China Agricultural University, 2014, 35(2): 90-95. DOI: 10.7671/j.issn.1001-411X.2014.02.017
    Citation: WANG Lu, DUAN Ranran, ZHAO Pan, ZHOU Xiaohua. Syntheses，characteristics，bacteriostatic activities and their interaction with DNA of copper(Ⅱ) complexes containing 2-aminomethylbenzimidazole[J]. Journal of South China Agricultural University, 2014, 35(2): 90-95. DOI: 10.7671/j.issn.1001-411X.2014.02.017
    shu

    含 2 - 氨甲基苯并咪唑铜(Ⅱ)配合物的合成、表征、抑菌活性及与 DNA 作用的研究

    Syntheses，characteristics，bacteriostatic activities and their interaction with DNA of copper(Ⅱ) complexes containing 2-aminomethylbenzimidazole

      摘要
    • 摘要: 【目的】为了设计和寻找DNA的特异性识别剂和断裂剂，合成了2 - 氨甲基苯并咪唑铜(Ⅱ)配合物：［Cu(AMB)2Cl］Cl·4H2O（配合物1）和［Cu(AMB)(phen)Cl］Cl·2H2O（配合物2）（AMB=2 - 氨甲基苯并咪唑，phen=1,10 - 邻菲咯啉）.【方法】通过元素分析、IR、UV和摩尔电导率对配合物进行了表征.用二倍稀释法测试了配合物对大肠埃希菌 Escherichia coil(G-）、沙门杆菌 Salmonella typhi(G-）、金黄色葡萄球菌 Staphylococcus aureus(G+)和枯草芽孢杆菌 Bacillus subtilis(G+)的最小抑菌浓度（MIC）.采用电子吸收光谱、荧光光谱、相对黏度及琼脂糖凝胶电泳法测试了2个配合物与 ct-DNA 的结合作用.【结果和结论】配合物1以非插入方式、配合物2以插入方式与 ct-DNA 作用；在 VC 存在下，2个配合物均通过·OH氧化机理切割 pBR322 DNA.2个配合物结合 ct-DNA 和切割 pBR322 DNA 的能力强弱均为：配合物2> 配合物1.

       

      Abstract: 【Objective】In order to design and find specific recognition and cleavage agent of DNA, two copper(Ⅱ) complexes,［Cu(AMB)2Cl］Cl·4H2O（complex 1）and［Cu(AMB)(phen)Cl］Cl·2H2O（complex 2）（AMB = 2-aminomethylbenzimidazole, phen=1,10-phenanthroline）, were synthesized. 【Method】The two complexes were characterized by elemental analysis, IR, UV and molar conductivity. The complex 1 and complex 2 were assayed against gram-positive bacteria (Staphylococcus aureus and Bacillus subtilis) and gram-negative bacteria(Escherichia coil and Salmonella typhi) by a double dilution method, and the interaction with ct-DNA was investigated by electronic absorption spectroscopy, fluorescence spectroscopy, viscosity measurements and agarose gel electrophoresis.【Result and conclusion】The complex 2 could interact with DNA by non-intercalation, while complex 1 by non-intercalation, and they cleave pBR322 DNA assisted by vitamin C involving the hydroxyl radical oxidation mechanism. The order of combination ability with ct-DNA and cleaving pBR322 DNA of the two complexes is complex 2＞complex 1, which is in accordance with the bacteriostatic activity.

       

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