Abstract: 【Objective】 This study aimed at isolating and screening of efficiently biological flocculation strains from the paddy field of the planted wild rice.【Method】A high bioflocculant strain YH39 was obtained from the screening of kaolin suspension flocculation effects of 135 strains isolated from Oryza officinalis on VM medium.【Result and conclusion】16S rDNA sequences analysis showed that the strain YH39 was closely related to the known species Burkholderia cepacia with 98.6% similarity. The best carbon and nitrogen sources for producing flocculation were glucose and potassium nitrate, respectively. The fermentation kinetics analysis showed that strain YH39 reached the highest values of biomass concentration and flocculating activity in 16 h. Flocculant composition analysis indicated that the bioflocculant components were polysaccharide with good heat resistance, which was heated at 60 ℃ for 30 min without losing flocculating activity. The application of bioflocculation of three kinds of dyeing wastewater show that the fermentation liquid has good flocculating effects on dyeing wastewater with effective decolorization and reduces COD_Cr in dyeing wastewater.