Abstract: In the present study, green fluorescent protein(GFP) transgenic embryos were reconstructed by nuclear transfer of GFP positive cells into enucleated in vitro matured oocytes. The development of reconstructed embryos both in vitro was observed, and GFP expression was checked as well. This research was conducted to study the processing of nuclear cells, the method of nuclear transfer and fusion/activation on early development in vitro of reconstructed embryos. The results showed that there were no significant differences in the cleavage rates between serum hunger and not hunger training 10 d using injection ways of perirenal space of eggs, and the cleavage rate of reconstructed embryos with intracytoplasmic injection (81.11%) was not significantly higher than that of the perirenal space of eggs (76.80%). After activation treatment, the rate of cleavage were assessed on 2 days, respectively. Although there were no significant differences in the cleavage rates of the first two treatment groups (75.61% and 83.07%), the developmental rate was significantly higher than that of the third group(60.00%).