Abstract: The aim of this paper is to conduct a bioinformatics analysis for p113 gene of Mycoplasma ovipneumoniae and further predict its functions. Using several softwares, homology of p113 gene with other genes was calculated and trans-membrane structure, repeat sequence, signal peptide and epitodes were predicted. The results revealed that p113 was an orthologous of Mycoplasma hyopneumoniae adhesin gene pp97 which shared similar R2 repeat sequence. Overall analysis leads to the prediction that P113 protein is one of the important adhesins and membrane immunogens.