Abstract: A method has been developed for the determination of dexynivalenol in edible tissues of swine by liquid chromatography coupled with electrospray ionization tandem mass spectrometry （LC-MS/MS）.The samples were extracted by pure water and purified by C₁₈ solid phase extraction, and then analyzed by the LC-MS/MS after redissolved by 30% methanol. The chromatographic column was Phenomenex Luna 5 μ C₁₈(2), and the flowing phase was comprised with methanol-0.1% formic acid solution (V/V=3∶7). The mass spectrometry was run with electrospray ion source and in positive ions multiple reaction monitoring mode for the quantitative and qualitative analyses. Limit of detection (LOD) was 25 μg·kg^-1, and limit of quantitation (LOQ) was 50 μg·kg^-1. The recoveries of dexynivalenol in edible tissues of swine ranged from 80.2% to 99.4% in 50, 100 and 500 μg·kg^-1 concentration levels. The relative standard deviation ranged from 2.47% to 9.47%.