杀虫双对杜氏盐藻生长和生理生化的影响
Effects of Chemical Pesticide Dimehypo on Growth, Physiological and Biochemical Characteristics of Dunaliella salina
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摘要: 以一种具有环境耐性的单细胞绿藻杜氏盐藻 Dunaliella salina 作为受试生物，检测一种杀虫剂杀虫双的毒性作用,试验中检测了杀虫双对杜氏盐藻细胞生长、单细胞β胡萝卜素质量、细胞形态变化及超氧化物歧化酶（Superoxide dismutase，SOD）和过氧化氢酶（Catalase, CAT）活性的影响.结果表明，当杀虫双质量浓度小于0.5 mg·L-1 时，细胞的毒性响应与对照的变化趋势类似.当使用1.0~4.0 mg·L-1杀虫双处理盐藻，处理初期细胞生长缓慢和单细胞β胡萝卜素质量降低，一段时间后恢复.当杀虫双质量浓度大于5.0 mg·L-1，细胞生长速度和单细胞β胡萝卜素质量都会一直降低直至检测不到.杀虫双对杜氏盐藻的10 d IC50为32 mg·L-1.试验发现在低浓度下杀虫双会刺激CAT活性的增长.Abstract: Dunaliella salina, a unicellular green alga with environmental tolerance, was employed as test organism to investigate the toxicity effects of a pesticide dimehypo. The influences of dimehypo on cell growth, single cell β-carotene level, cell morphology changes, and activities of superoxide dismutase (SOD) and catalase (CAT) were investigated. At the mass concentrations less than 0.5 mg·L-1 dimehypo, cell responses were similar to control. When treated with 1.0-4.0 mg·L-1 dimehypo, cell growth and single cell β-carotene levels declined at first and then revived. When mass concentrations were higher than 5.0 mg·L-1 dimehypo, both cell growth and single cell β-carotene levels decreased until they were undetectable. The 10 d IC50 of dimehypo on D. salina was 32 mg·L-1. Dimehypo could stimulate the increase of CAT activity at a low concentration.
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Keywords:
- Dunaliella salina /
- toxicity /
- dimehypo
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