Abstract: In order to study the application of aroma substances GC/MS fingerprint in the quality control of flue-cured tobacco, the Nanping flue-cured tobaccoes were grouped into 3 grades—medium, medium+ and better—according to the sensory evaluation, and the corresponding standard fingerprints of the three grades flue-cured tobacco were established. The fingerprint similarity of each sample with standard fingerprint was calculated. The high values indicated the high similarity, threshold of the three grades were defined according to the similarity of the fingerprint, which were 0.731 2, 0.703 6 and 0.676 7 respectively. Pattern recognization of the three grades flue-cured tobacco was carried out by projection drawing of principal constituent analysis. Pattern recognization results showed the good spatial distribution in the chart, obvious section, which indicated the feasibility and resolution of the quality control system.The unknown samples were used to test the effectiveness of the quality control system. The result indicated that the system had high accuracy, low error rate, and adaptability to the quality control of flue-cured tobacco.