(2-(4′-噻唑基)苯并咪唑)(<i>L</i>-丙氨酸根)铜(Ⅱ)配合物的合成、表征、抑菌活性及与DNA的作用

朱 丽; 卢艳梅; 区志镔; 乐学义

doi:10.7671/j.issn.1001-411X.2012.04.029

(2-(4′-噻唑基)苯并咪唑)(L-丙氨酸根)铜(Ⅱ)配合物的合成、表征、抑菌活性及与DNA的作用

Synthesis, Characterization, Antibacterial Activities and DNA Interaction of Ternary Copper(Ⅱ) Complex with 2-(4′-Thiazolyl)Benzimidazole and L-Alaninate

摘要

摘要: 合成了新的三元铜(Ⅱ)配合物：［Cu(TBZ)(L-Ala)(H₂O)］ClO₄［其中，TBZ=2-(4′-噻唑基)苯并咪唑，L-Ala=L-丙氨酸根］．通过元素分析、摩尔电导率、红外光谱及电子吸收光谱对该配合物进行了表征．用试管二倍稀释法研究了配合物的抗菌活性，发现配合物对金黄色葡萄球菌Staphylococcus aureus(G+)、枯草杆菌Bacillus subtilis(G+)、沙门氏杆菌Salmonella typhi(G-)和大肠埃希菌Escherichia coil(G-)具有良好的抑制作用．另外，采用电子吸收光谱、荧光光谱、圆二色光谱、粘度测定及琼脂凝胶电泳方法研究了配合物与小牛胸腺DNA(CT-DNA)的作用．结果表明，配合物可能以插入方式与CT-DNA作用，在维生素C存在下通过羟自由基(·OH)，单线态氧(¹O₂)或者¹O₂类似物切割pBR322 DNA双螺旋结构．

Abstract: A new ternary copper(Ⅱ) complex: ［Cu(TBZ)(L-Ala)(H₂O)］ClO₄［TBZ=2-(4′-thiazolyl) benzimidazole, L-Ala =L-alaninate］, was synthesized and characterized by elemental analysis, molar conductivity, infrared absorption spectrum, and electronic absorption spectrum. The complex was assayed against gram-positive(Staphylococcus aureus, Bacillus subtilis) and gram-negative(Salmonella typhi, Escherichia coil) bacteria by double dilution method, and the interaction of the complex to DNA was investigated by electronic absorption spectrometry, fluorescence spectrometry, CD spectrometry, viscosity measurement and agarose gel electrophoresis. The results indicated that the complex had good antibacterial activity, which could be bound to CT-DNA (calf thymus DNA) by intercalative mode, cleave pBR322 DNA in the presence of vitamin C in the involvement of the hydroxyl radical, and possibly singlet oxygen.

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