Abstract: In order to investigate influence of DHA compound additives in diets on egg quality and production performance, 96 Hy-Line layers were divided into 4 groups at random, and each group consisted of 6 cages with 4 laying hens per cage. GroupⅠ was control group and fed with basal diet. Groups Ⅱ-Ⅳ were experimental groups and fed increasing levels of DHA compound additives at 2%,4% and 8% respectively. Results showed that average laying rate, average egg shape index and average feed/egg ratio of all groups were not significantly different(P>0.05); the average egg mass of groups Ⅱ-Ⅳ were found to be significantly higher than that of groupⅠ(P<0.01) in the whole trial period. Average haugh unit of group Ⅳ was also significantly higher than that of group Ⅰ in the first two weeks (P<0.01). In the third week, average haugh unit of group Ⅳ was significantly higher than that of the other three groups (P<0.05). In the last two weeks, average contents of DHA in groups Ⅱ-Ⅳ were significantly higher than those of group Ⅰ(P<0.01), whereas group Ⅳ was the highest. To sum up, the effects of DHA compound additive in diets can improve production performance and egg quality. According to the experiment results, the 8% DHA compound additive in diets is found to be the best approach.