短颈剑线虫和喜马拉雅剑线虫的形态与rDNA分子特征

Morphological and Molecular Characterization of Xiphinema brevicollum and X. himalayense

摘要: 描述了日本进境罗汉松根际截获的2种美洲剑线虫：短颈剑线虫 Xiphinema brevicollum Lordello et Da Costa,1961和喜马拉雅剑线虫 Xiphinema himalayense Ahmad，Lamberti, Rawat, Agostinelli et Srivastava, 1998，并对这2种线虫rDNA-ITS和D2D3序列进行了扩增、克隆、测序及系统进化分析.短颈剑线虫和喜马拉雅剑线虫在国家质量监督检验检疫局内首次截获，喜马拉雅剑线虫在日本首次报道，在中国没有分布记载.

Abstract: Morphological and molecular characterization of two putative Xiphinema americanum-group species (X. brevicollum and X. himalayense) from Yaccatree imported from Japan were described. ITS and D2D3 segments of two species were amplified, cloned, sequenced and analysed by using ME polygenetic tree. They were first intercepted by the General Administration of Quality Supervision Inspection and Quarantine of the People’s Republic of China. X. himalayense was reported in Japan and has no distribution record in China.