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    肖熙鸥, 王勇, 李冠男, 曹必好, 雷建军, 陈国菊, 陈清华. 茄子种质资源的ISSR遗传多样性分析[J]. 华南农业大学学报, 2012, 33(3): 296-300. DOI: 10.7671/j.issn.1001-411X.2012.03.005
    引用本文: 肖熙鸥, 王勇, 李冠男, 曹必好, 雷建军, 陈国菊, 陈清华. 茄子种质资源的ISSR遗传多样性分析[J]. 华南农业大学学报, 2012, 33(3): 296-300. DOI: 10.7671/j.issn.1001-411X.2012.03.005
    shu
    XIAO Xi-ou, WANG Yong, LI Guan-nan, CAO Bi-hao, LEI Jian-jun, CHEN Guo-ju, CHEN Qing-hua. ISSR Analysis of Genetic Diversity Among Cultivated Eggplant and Wild Relatives[J]. Journal of South China Agricultural University, 2012, 33(3): 296-300. DOI: 10.7671/j.issn.1001-411X.2012.03.005
    Citation: XIAO Xi-ou, WANG Yong, LI Guan-nan, CAO Bi-hao, LEI Jian-jun, CHEN Guo-ju, CHEN Qing-hua. ISSR Analysis of Genetic Diversity Among Cultivated Eggplant and Wild Relatives[J]. Journal of South China Agricultural University, 2012, 33(3): 296-300. DOI: 10.7671/j.issn.1001-411X.2012.03.005
    shu

    茄子种质资源的ISSR遗传多样性分析

    ISSR Analysis of Genetic Diversity Among Cultivated Eggplant and Wild Relatives

      摘要
    • 摘要: 利用ISSR分子标记分析茄子种质多样性，从100条引物中筛选出20条能够扩增出多态性条带稳定清晰的ISSR引物,对48份茄子材料的DNA进行扩增，共获得了186条谱带，其中多态性带153条（占所有谱带的82.2%）.利用UPGMA法建立了48份茄子及其近缘野生种间的遗传关系聚类图.聚类结果表明，48份供试种质间的遗传相似系数分布在0.52~0.98之间，可将其分为5大类群.其中又将44份栽培品种分为5组，其品种间相似遗传系数在0.84~0.98之间，表明栽培品种的遗传背景比较窄.聚类结果与茄子的果形以及地理来源有一定的相关性，而与果色呈现不完全相关.

       

      Abstract: The genetic relationship and diversity of 44 eggplants, Solanum melongena L., germplasms and 4 wild relatives were analyzed by ISSR method .One hundred and fifty-three of 186 bands were amplified by 20 ISSR primers, about 82.2% being polymorphisms. Fourty-eight germplasms were clustered into 5 groups by genetic similar index from 0.52 to 0.98, and the wild species were different from eggplants. Also 44 eggplant cultivars had a high genetic similarity and a narrow genetic diversity. At the same time, the results showed that the cluster was related to the fruit shape of eggplants and origin areas, but not to the fruit colour.

       

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