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    黄献培,罗建军,胡美英,梁颖仪,叶欣欣,钟国华. 肇庆鱼藤内生真菌的分离及生物活性研究[J]. 华南农业大学学报, 2009, 30(2). DOI: 10.7671/j.issn.1001-411X.2009.02.012
    引用本文: 黄献培,罗建军,胡美英,梁颖仪,叶欣欣,钟国华. 肇庆鱼藤内生真菌的分离及生物活性研究[J]. 华南农业大学学报, 2009, 30(2). DOI: 10.7671/j.issn.1001-411X.2009.02.012
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    HUANG Xian-pei,LUO Jian-jun,HU Mei-ying,LIANG Ying-yi,YE Xin-xin,ZHONG Guo-hua. Isolation and Bioactivity of Endophytic Fungi in Derris hancei[J]. Journal of South China Agricultural University, 2009, 30(2). DOI: 10.7671/j.issn.1001-411X.2009.02.012
    Citation: HUANG Xian-pei,LUO Jian-jun,HU Mei-ying,LIANG Ying-yi,YE Xin-xin,ZHONG Guo-hua. Isolation and Bioactivity of Endophytic Fungi in Derris hancei[J]. Journal of South China Agricultural University, 2009, 30(2). DOI: 10.7671/j.issn.1001-411X.2009.02.012
    shu

    肇庆鱼藤内生真菌的分离及生物活性研究

    Isolation and Bioactivity of Endophytic Fungi in Derris hancei

      摘要
    • 摘要: 从肇庆鱼藤Derris hancei分离获得内生真菌10属16株,体外拮抗作用测定表明,鱼藤茎内生菌株Penicillium sp.Q1、Rhizoctonia sp.S1、Phomopsis sp.N2、Corticium sp.F1和叶内生菌株Penicillium sp.Q2对芒果炭疽病菌Colle-totrichum gloeosporioides、西瓜枯萎病菌Fusarium oxysporum f.niveum Rhizoctonia sp.S1对黄瓜炭疽病菌Colletotri-chum orbiculare以及Phomopsis sp.N2对香蕉炭疽病菌Colletotrichum musae的对峙培养拮抗系数均达到Ⅱ级,且证实鱼藤内生真菌可产生抑菌活性物质.浸叶法测定表明,叶内生菌Penicillium sp.Q2菌株发酵液活性最强,处理后48 h,斜纹夜蛾Spodoptera litura 2龄幼虫和萝卜蚜Lipaphis erysimi无翅成蚜校正死亡率分别为100.00%和75.10%,活性高于其他分离筛选所获菌株.

       

      Abstract: Sixteen endophytic fungi belonging 10 genus were isolated from Derris hancei Hemsl.The antagonism of endophytic fungi against fungal pathogens was tested in vitro.Penicillium sp.Q1,Rhizoctonia sp.S1,Phomopsis sp.N2,and Corticium sp.F1 isolated from the caudex of D.hancei,and Penicillium sp.Q2 isolated from the leaf,inhibited the hyphal growth of Colletotrichum gloeosporioides Penz,Fusarium oxysporum f.niveum(E.F.Smith)Snyber et Hansen,Rhizoctonia sp.S1 against Colletotrichum orbiculare Arx,and Phomopsis sp....

       

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