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    赵志权,王福广,郝环颍,张思远,贺东生. 高致病性猪繁殖与呼吸综合征病毒的RT-PCR鉴别诊断方法[J]. 华南农业大学学报, 2008, 29(3). DOI: 10.7671/j.issn.1001-411X.2008.03.020
    引用本文: 赵志权,王福广,郝环颍,张思远,贺东生. 高致病性猪繁殖与呼吸综合征病毒的RT-PCR鉴别诊断方法[J]. 华南农业大学学报, 2008, 29(3). DOI: 10.7671/j.issn.1001-411X.2008.03.020
    shu
    ZHAO Zhi-quan,WANG Fu-guang,HAO Huan-ying,ZHANG Si-yuan,HE Dong-sheng. Diagnosis and Identification of the Highly Virulent Porcine Reproductive and Respiratory Syndrome Virus by RT-PCR[J]. Journal of South China Agricultural University, 2008, 29(3). DOI: 10.7671/j.issn.1001-411X.2008.03.020
    Citation: ZHAO Zhi-quan,WANG Fu-guang,HAO Huan-ying,ZHANG Si-yuan,HE Dong-sheng. Diagnosis and Identification of the Highly Virulent Porcine Reproductive and Respiratory Syndrome Virus by RT-PCR[J]. Journal of South China Agricultural University, 2008, 29(3). DOI: 10.7671/j.issn.1001-411X.2008.03.020
    shu

    高致病性猪繁殖与呼吸综合征病毒的RT-PCR鉴别诊断方法

    Diagnosis and Identification of the Highly Virulent Porcine Reproductive and Respiratory Syndrome Virus by RT-PCR

      摘要
    • 摘要: 为了快速鉴别诊断高致病性猪繁殖与呼吸综合症,参照GeneBank已发表的猪繁殖与呼吸综合征病毒(PRRSV)的Nsp2基因,设计了1对引物,建立了一种RT-PCR检测方法.该方法可以区分高致病性PRRSV毒株与经典PRRSV弱毒疫苗株,扩增PCV2、PRV、PPV和CSFV等均为阴性.该方法敏感性较高,可以检测到0.2 ng的病毒核酸.

       

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