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    何逸民,孔留五,罗玉均,潘全会,曹宗喜,张桂红. 2株猪圆环病毒2型分离毒株全基因组的克隆及序列分析[J]. 华南农业大学学报, 2008, 29(3). DOI: 10.7671/j.issn.1001-411X.2008.03.019
    引用本文: 何逸民,孔留五,罗玉均,潘全会,曹宗喜,张桂红. 2株猪圆环病毒2型分离毒株全基因组的克隆及序列分析[J]. 华南农业大学学报, 2008, 29(3). DOI: 10.7671/j.issn.1001-411X.2008.03.019
    shu
    HE Yi-min,KONG Liu-wu,LUO Yu-jun,PAN Quan-hui,CAO Zong-xi,ZHANG Gui-hong. Molecular Cloning, Sequencing and Analysis of the Complete Genome of two Porcine Circovirus Type 2 Isolates[J]. Journal of South China Agricultural University, 2008, 29(3). DOI: 10.7671/j.issn.1001-411X.2008.03.019
    Citation: HE Yi-min,KONG Liu-wu,LUO Yu-jun,PAN Quan-hui,CAO Zong-xi,ZHANG Gui-hong. Molecular Cloning, Sequencing and Analysis of the Complete Genome of two Porcine Circovirus Type 2 Isolates[J]. Journal of South China Agricultural University, 2008, 29(3). DOI: 10.7671/j.issn.1001-411X.2008.03.019
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    2株猪圆环病毒2型分离毒株全基因组的克隆及序列分析

    Molecular Cloning, Sequencing and Analysis of the Complete Genome of two Porcine Circovirus Type 2 Isolates

      摘要
    • 摘要: 参考GenBank发表的猪圆环病毒2型(PCV2)全基因组序列,设计2对引物,通过PCR技术扩增出2株PCV2流行株的全基因,并进行了序列测定分析.结果表明,2个分离株基因组全长分别为1 767 bp和1 768 bp,2个分离株之间的核苷酸相似性为97.3%,与GenBank上已发表的PCV2分离株之间的相似性为93.8%～99.3%.2个分离株的ORF2核苷酸序列相似性分别为91.3%～99.6%和90.6%～98.0%,存在一定的变异.

       

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