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    杨金广,方振兴,张孟倩,徐飞,王文婷,谢荔岩,林奇英,吴祖建,谢联辉. 应用Real-Time RT-PCR鉴定2个水稻品种(品系)对水稻条纹病毒的抗性差异[J]. 华南农业大学学报, 2008, 29(3). DOI: 10.7671/j.issn.1001-411X.2008.03.006
    引用本文: 杨金广,方振兴,张孟倩,徐飞,王文婷,谢荔岩,林奇英,吴祖建,谢联辉. 应用Real-Time RT-PCR鉴定2个水稻品种(品系)对水稻条纹病毒的抗性差异[J]. 华南农业大学学报, 2008, 29(3). DOI: 10.7671/j.issn.1001-411X.2008.03.006
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    YANG Jin-guang,FANG Zhen-xing,ZHANG Meng-qian,XU Fei,WANG Wen-ring,XIE Li-yan,LIN Qi-ying,WU Zu-jian,XIE Lian-hui. Detection on Different Resistance of Two Rice Varieties Against Rice stripe virus in Real-Time RT-PCR[J]. Journal of South China Agricultural University, 2008, 29(3). DOI: 10.7671/j.issn.1001-411X.2008.03.006
    Citation: YANG Jin-guang,FANG Zhen-xing,ZHANG Meng-qian,XU Fei,WANG Wen-ring,XIE Li-yan,LIN Qi-ying,WU Zu-jian,XIE Lian-hui. Detection on Different Resistance of Two Rice Varieties Against Rice stripe virus in Real-Time RT-PCR[J]. Journal of South China Agricultural University, 2008, 29(3). DOI: 10.7671/j.issn.1001-411X.2008.03.006
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    应用Real-Time RT-PCR鉴定2个水稻品种(品系)对水稻条纹病毒的抗性差异

    Detection on Different Resistance of Two Rice Varieties Against Rice stripe virus in Real-Time RT-PCR

      摘要
    • 摘要: 应用Real-time RT-PCR检测了水稻条纹病毒(Rice stripe virus,RSV)在2种水稻品种(品系)武育粳3号和KT95-418的悬浮细胞内复制变化和相对含量的差异,结合传统生物学接种试验,确定了这2个品种(品系)对RSV抗性的差异.结果表明,RSV在武育粳3号的悬浮细胞内24 h达到复制高峰,病毒含量为侵染初期的7.46倍.而在KT95-418的悬浮细胞内,RSV达到复制高峰需要36 h,病毒含量为侵染初期的4.51倍.利用病毒生物学接种的方法,武育粳3号发病率达91.7%,而KT95-418仅为36.0%.由此可见,KT95-418较武育粳3号对RSV具有较高的抗病性.因此,Real-time RT-PCR方法与传统生物学接种试验方法相比,具有更高的准确性和灵敏性,可以作为传统品种抗病性鉴定的验证手段.

       

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