Abstract: To study the characteristics of rabies virus as vector, hemagglutinin gene of avian influenza virus was inserted at the site of pseudogene between G and L genes and a chimeric virus, designated as HepFHA, was generated by the technique of reverse genetics. The virus HepFHA grew well in BHK-21 cells after passage for 10 times, but did not show hemagglutination. The foreign protein was analyzed by SDS-PAGE and Western-blotting, and the results indicated that the partial protein of hemagglutinin was expressed with relative molecular mass of 39 540, identical to that of HA1.