Abstract: Twenty species of plants collected from Three Gorges region,Hubei Province,were screened for their antifungal activity against six important plant pathogenic fungi: Peronophthora litchii,Phytophthora melonis,Magnaporthe grisea,Rhizoctonia solani,Fusarium oxysporum f.sp.niveum,and Colletotrichum musae,in vitro.The plants were dried,ground,and then extracted with methanol.The dry methanol extracts were tested for their inhibition of mycelial growth on PDA plates at mass concentration of dry methanol extract 10 mg/mL.The results showed that the inhibitory rate to P.litchii in methanol extracts from Melampyrum roseum,Dendranthema vestitum and Hamamelis mollis were 100%,100%,and 82.35%,respectively,while that to P.melonis in methanol extracts from M.roseum,D.vestitum and Caryopteris incana were 100%,96.23%,and 82.43%,respectively.In case of M.grisea,higher inhibitory rate occurred from H.mollis(61.51%) and Machilus ichangensis(61.51%),while Phryma leptostachya and Vicia unijuga exhibited higher inhibitory rate to R.solani,which were 78.48% and 78.03%,respectively.The effect of H.mollis showed the highest inhibitory rate to both of F.oxysporum f.sp.niveum and C.musae,which were 51.12% and 61.07%,respectively.The inhibitory rate in total plant dry mass was discussed in the paper.