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    吴丽君,胡开林,吴智明. 黄瓜花叶病毒外壳蛋白基因转化辣椒的研究[J]. 华南农业大学学报, 2007, 28(2): 69-72. DOI: 10.7671/j.issn.1001-411X.2007.02.017
    引用本文: 吴丽君,胡开林,吴智明. 黄瓜花叶病毒外壳蛋白基因转化辣椒的研究[J]. 华南农业大学学报, 2007, 28(2): 69-72. DOI: 10.7671/j.issn.1001-411X.2007.02.017
    shu
    WU Li-jun,HU Kai-lin,WU Zhi-ming. Transformation of Capsicum annuum with Coat Protein Gene of Cucumber Mosaic Virus[J]. Journal of South China Agricultural University, 2007, 28(2): 69-72. DOI: 10.7671/j.issn.1001-411X.2007.02.017
    Citation: WU Li-jun,HU Kai-lin,WU Zhi-ming. Transformation of Capsicum annuum with Coat Protein Gene of Cucumber Mosaic Virus[J]. Journal of South China Agricultural University, 2007, 28(2): 69-72. DOI: 10.7671/j.issn.1001-411X.2007.02.017
    shu

    黄瓜花叶病毒外壳蛋白基因转化辣椒的研究

    Transformation of Capsicum annuum with Coat Protein Gene of Cucumber Mosaic Virus

      摘要
    • 摘要: 以辣椒品种'B162'为试材,探讨了通过根癌农杆菌介导法将黄瓜花叶病毒外壳蛋白(CMV-CP)基因转化辣椒的适宜条件.结果表明,将辣椒带柄子叶进行预培养2 d后,用D600 nm为0.3的根癌农杆菌菌液浸泡7 min,再经过共培养2 d后转移到筛选分化培养基中进行培养,有利于获得辣椒抗性不定芽.通过对获得的10株抗性不定芽进行PCR检测,其中有2株扩增出目的条带,初步证明CMV-CP基因已经转入辣椒不定芽中.

       

      Abstract: Agrobacterium-mediated transformation of pepper 'B162'with coat protein gene of cucumber mosaic virus(CMV-CP) was studied.Results showed that the best transformation system was: cotyledons explants were precultured for 2 d,and then inoculated with Agrobacterium tumefaciens(D600 nm= 0.3) for 7 min,transferred onto selection differentiation medium after co-cultured for 2 d.10 green adventitious buds with Kan-resistance were screened by Polymerase chain reaction.Two of them were amplified with the band of the fragment of 740 bp.The result showed the CMV-CP gene had been incorporated into the plant genome successfully.

       

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