复合微生物菌剂对番茄青枯病和土壤微生物多样性的影响
Effects of Complex Microbial Fertilizer on Tomato Bacterial Wilt and Soil Microbial Diversities
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摘要: 采用微生物培养和BIOLOG GN2微平板培养,研究了施入自制的复合微生物菌剂对番茄青枯病和土壤微生物多样性的影响.平板抑菌试验结果表明,复合菌剂对番茄青枯病菌有一定的拮抗作用;盆栽试验结果显示,施入复合菌剂后,可延缓青枯病发病时间并降低发病率;不同程度地提高了土壤微生物群落的平均每孔颜色变化率(AWCD)和多样性指数;主成分分析表明,施入复合菌剂的处理,在番茄生长中期,土壤微生物群落功能较相似,其菌群特征是对糖类和聚合物优先利用,对羧酸类利用率则较低.Abstract: Effects of complex microbial fertilizer on tomato bacterial wilt and soil microbial diversities were studied by agar media test and BIOLOG GN micro-plate culture.Agar medium antagonistic test showed that pure culture of Ralstonia solanacearum were inhibited by compound microbial fertilizer.The pot experiments indicated that the complex microbial fertilizer delayed the disease occurrence time and reduced the disease severity in infested and CK soil,and increased the average well color development(AWCD) and diversity index.The principal component analysis showed that functional diversity of soil microbial community treated with compound microbial fertilizer shift to disease-resistance.The components of carbonhydrate and polymer were prior to utilize by soil microbe,while utilization efficiency of carboxylic was low.
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