Abstract: This study has determined the genetic relations between the phenomenon of significant asymmetric ratio of the two component nuclear types from dikaryons and cytoplasm factors in Lentinula edodes via di-mon mating. Genetic analysis to fully compatible di-mon mating showed that the monokaryons with rived dikaryon, this showed that a pre-entrance nucleus exists in donor and pre-entrance nucleus mainly dependent on the compatible specificity of B mating-type factor between acceptor and donor nucleus; the higher the specificity, the faster the migration of pre-entrance nucleus.