猪口蹄疫病毒DNA疫苗与常规灭活苗的比较
Comparison of Effects of FMDV DNA Vaccines and Conventional Vaccine
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摘要: 以含有FMDV VPI基因和VPI基因主要抗原位点141—160及200—213位的氨基酸的真核表达质粒pcDNA-VPI和pcDNA-F与常规O型FMD灭活疫苗分别免疫6—8周龄的BALB／c小鼠和豚鼠，同时设生理盐水为阴性对照，以肌肉注射，间隔2周3次免疫的方式进行．通过脾脏T淋巴细胞增殖（MTT）、T淋巴细胞亚群、中和抗体、抗病毒能力的检测和病理组织学观察等方面比较DNA疫苗与常规灭活苗的免疫效果．结果发现pcDNA-VPI和pcDNA-F能够诱导细胞免疫和体液免疫反应，pcDNA-F可使小鼠和豚鼠产生一定程度的抗FMDV感染的保护性免疫，但与常规灭活苗比较还存在差异．Abstract: six to eight-week-old BALB/c mice and guinea pigs were immunized with eukaryotic plasmids and normal FMDV type O vaccine by intramuscular injection, respectively for three times, and immunization interval was two weeks. The F fragment codes for the amino acids at sites 140-160 and 200-213, the major antigenic epitopes of FMDV type O. The immune effects of DNA vaccines were compared with that of conventional vaccine at MTT, T lymphcyte subgroup, neutralizing antibody, resistance to FMDV and histopathology. The results showed that DNA vaccines pcDNA-VPI and pcDNA-F could induce humoral and cellular immunity. BALB/c mice and guinea pigs immuned with pcDNA-F could were resistant to FMDV, but there were difference with conventional vaccine.
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Keywords:
- foot-and-mouth disease virus /
- VPI gene /
- antigenic epitopes /
- plasmid
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