Abstract: An experiment was conducted, for Saccharopolyspora spinosa in the changes of biomass, residual carbohydrate content, spinosad content, the pH value in the cultural medium by the shaking flash. The kinetic models of batch fermentation by S. spinosa for biomass, substrate consumption and spinosad production were studied. The models were fitted and provided a useful aquation for biomass, substrate consumption and spinosad production. And the parameters of the models were obtained by using non-linear regretssion techniques simulated by MATLAB software. The temporal regulations of biomass, residual carbohydrate,spinosad content were gotten in the batch fermention. The correlation coefficients of the models were 0.98934,0. 99266,0. 98635 respectively. The fitted models could reflect the batch fermentation process of S. spinosa.