Abstract: Protein and oil content of different soybean cultivars was determined by using a near infrared Grain Analyzer. The result showed that there was no significant difference in protein and oil content between low and high phosphorus treatments. The protein content was negatively correlated with the oil content but positively correlated with the total content of protein and oil. Under the high P treatment, the 14.09% of soybean cuhivars had more than 63% of total protein and oil content; while under the low P treatment,the 15.45% of soybean cultivars had also more than 63% of total protein and oil content. Therefore, these cultivars will benefit the breeding of soybean varieties with high-protein and high-oil content.