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    罗琴芳,刘华圆,郭霄峰. 野鸡源H9N2亚型禽流感病毒NA基因的序列分析[J]. 华南农业大学学报, 2006, 27(1): 101-103. DOI: 10.7671/j.issn.1001-411X.2006.01.026
    引用本文: 罗琴芳,刘华圆,郭霄峰. 野鸡源H9N2亚型禽流感病毒NA基因的序列分析[J]. 华南农业大学学报, 2006, 27(1): 101-103. DOI: 10.7671/j.issn.1001-411X.2006.01.026
    shu
    LUO Qing-fang,LIU Hua-yuan,GUO Xiao-feng. Analysis of NA Gene of H9N2 Avian Influenza Virus from Pheasant[J]. Journal of South China Agricultural University, 2006, 27(1): 101-103. DOI: 10.7671/j.issn.1001-411X.2006.01.026
    Citation: LUO Qing-fang,LIU Hua-yuan,GUO Xiao-feng. Analysis of NA Gene of H9N2 Avian Influenza Virus from Pheasant[J]. Journal of South China Agricultural University, 2006, 27(1): 101-103. DOI: 10.7671/j.issn.1001-411X.2006.01.026
    shu

    野鸡源H9N2亚型禽流感病毒NA基因的序列分析

    Analysis of NA Gene of H9N2 Avian Influenza Virus from Pheasant

      摘要
    • 摘要: 通过病毒RNA抽提、RT-PCR、克隆，试验一次性获得野鸡源禽流感病毒A／Pheasant／Guangdong／GZI／2003（H9N2）NA全基因．序列分析及联机检索表明，该病毒的NA基因由1458bp组成，其中开放阅读框（ORF）l401bp编码466个氨基酸；ORF与A／Chicken／Guangdong／11／97同源性最高，达98％；与A／Chicken／Guangdong／11／97的NA亲缘性最近．

       

      Abstract: Based on the reported sequences of NA gene of avian influenza virus(AIV) H9N2 subtype,a pair of primers was designed for the amplification of NA gene from a strain of AIV H9N2 subtype isolated from a pheasant(A/Pheasant /Guangdong /GZI/2003(H9N2) by RT-PCR.Sequence analysis indicated that the NA gene was 1 458 bp and the open reading frame(ORF) was 1 401 bp in length,encoding for 466 amino acids,sharing more than 98% identities with A/Chicken/Guangdong/11/97(H9N2).

       

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