Abstract: The differentiation of three haplotypes of Bemisia tabaci populations in China was analyzed using RAPD-PCR in this study.Five B.tabaci populations were collected in the north,middle and south of China,respectively,including three B biotype populations,the indigenous haplotype populations(NaC haplotype) and a new haplotype population from ornamental plants(C_v haplotype).In addition,the greenhouse whitefly,Trialeurodes vaporariorum,was used as a outgroup population in this analysis.The results of the cluster analysis using genetic distances indicated that,first,the population relationship between the NaC haplotype and the C_v haplotype was more close than that between B biotype and NaC haplotype,or that between B biotype and the C_v haplotype;second,the population relationships of the three haplotype of B.tabaci in China was intraspecific,and the C_v haplotype was potentially originated in China or the neighboring countries.