香蕉束顶病毒复制酶和黄瓜花叶病毒衣壳蛋白融合基因植物表达载体的构建

Construction of plant expression vector of fusion genes with Banana bunchy top virus replicase and Cucumber mosaic virus coat protein

摘要: 利用重组PCR技术将香蕉束顶病毒的复制酶基因和黄瓜花叶病毒的衣壳蛋白基因融合重组，并与植物表达载体pBI121连接，构建了抗香蕉束顶病和香蕉花叶病的双价植物表达载体，通过冻融法转化载体人根癌农杆菌LBA4404，获得了工程农杆菌pBI121．FBC，从而为培育抗病转基因香蕉品种奠定了基础．

Abstract: Two genes, the replicase gene from Banana bunchy top virus and coat protein gene from Cucumber mosaic virus, were firstly fused together with PCR, and were inserted into a plant expression vector-pBI121. The plant expression vector was transformed to Agrobacterium tumefaciens LBA4404. As a result, the engineered agrobacteria containing pBI121.FBC were gotten, and would be further used to develop a engineering banana variety resistant to two kinds of viruses.