木瓜凝乳蛋白酶的自水解作用及其对分离纯化的影响

The autolysis of chymopapain and the effect on its purification

摘要: 对木瓜凝乳蛋白酶的自水解作用及其对分离纯化的影响作了探讨．结果表明：激活的和未激活的木瓜凝乳蛋白酶液均能发生自水解作用，而激活剂的使用可加速酶的自水解过程，在分离纯化过程中易造成分离纯化产物不均一、酶活力回收率和蛋白回收率下降等；与之相比，未激活的木瓜凝乳蛋白酶的应用可抑制或延缓分离过程中酶的水解，纯化产物蛋白回收率达64．08％，比前者提高了10％以上；酶活力回收达68．9％，比前者提高了15％。

Abstract: The effect of chymopapain autolysis on its purification was studied. It was found that: activated and inactivated chymopapain solution could undergo autolysis. The use of activator accelerated the autolytic process, resulting in heterogeneity of the purified products, and decline in the recovery of enzyme activity and protein. By contrast, using inactivated chymopapain inhibited or delayed the autolysis, the recovery of protein was 64.08%, showing an increase of more than 10%, and recovery of activity was 68.9%, a gain of over 15%.