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    饶雪琴 张曙光 高乔婉. 工厂化组培香蕉分化芽中病毒的检测[J]. 华南农业大学学报, 2005, 26(1): 64-66. DOI: 10.7671/j.issn.1001-411X.2005.01.017
    引用本文: 饶雪琴 张曙光 高乔婉. 工厂化组培香蕉分化芽中病毒的检测[J]. 华南农业大学学报, 2005, 26(1): 64-66. DOI: 10.7671/j.issn.1001-411X.2005.01.017
    shu
    RAO Xue-qin,ZHANG Shu-guang,GAO Qiao-wan. Detecting viruses in banana differentiated shoots from tissue culture[J]. Journal of South China Agricultural University, 2005, 26(1): 64-66. DOI: 10.7671/j.issn.1001-411X.2005.01.017
    Citation: RAO Xue-qin,ZHANG Shu-guang,GAO Qiao-wan. Detecting viruses in banana differentiated shoots from tissue culture[J]. Journal of South China Agricultural University, 2005, 26(1): 64-66. DOI: 10.7671/j.issn.1001-411X.2005.01.017
    shu

    工厂化组培香蕉分化芽中病毒的检测

    Detecting viruses in banana differentiated shoots from tissue culture

      摘要
    • 摘要: 采用间接ELISA法和PCR方法对组织培养的香蕉分化芽中黄瓜花叶病毒(CMV)、香蕉线条病毒(BSV)和香蕉束顶病毒(BBTV)分别进行了检测，用间接ELISA法抽检香蕉分化芽中的CMV，带毒率为2．5％左右；PCR法检测香蕉分化芽中：BSV和BBTV、40个送检样品中带BSV的为12．5％，22个送检样品中未检测到带BBTV的香蕉分化芽。

       

      Abstract: Indirect ELISA and PCR methods were used to detect the existence of cucumber mosaic virus (CMV), banana streak virus (BSV) and banana bunchy top virus (BBTV) respectively in cultured differentiated banana shoots, the results showed that the rate of CMV-carriers was about 2.5% by indirect ELISA, the rate of BSV-carriers was 12.5% of 40 samples by PCR, and no BBTV-carrier was found in 22 samples by PCR.

       

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