Abstract: The relationship between air anion concentrations at different plots and environment factors was analyzed based on a survey of the air anion concentrations in Chebaling National Nature Reserve. The results showed that brook surrounding, waterfall surrounding and near-water place had higher air anion concentrations. There is a significant relation between forest soil, vegetation and the air anion concentrations. Health trails were suggested to be built in places with higher air anion concentrations for recreation and healthcare.