Abstract: The myostatin gene of chicken and goose was amplified by reverse transcription-polymerase chain reaction(RT-PCR),then cloned into pGEM-T Easy vector and sequenced separately. The result showed that the complete gene either obtained from chicken or goose skeletal muscle consisted of (1 128) nucleotides, chicken myostatin cDNA sequence shared 94.6% homology with goose myostatin cDNA sequence,and the homology of myostatin amino acids sequence between them was 97.9%, which was higher than that of the cDNA sequence.