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    马现永 施振旦 曹永长 毕英佐 马静云. 鸡、鹅肌肉生成抑制因子基因的克隆及序列分析[J]. 华南农业大学学报, 2004, 25(2): 85-88. DOI: 10.7671/j.issn.1001-411X.2004.02.022
    引用本文: 马现永 施振旦 曹永长 毕英佐 马静云. 鸡、鹅肌肉生成抑制因子基因的克隆及序列分析[J]. 华南农业大学学报, 2004, 25(2): 85-88. DOI: 10.7671/j.issn.1001-411X.2004.02.022
    shu
    MA Xian-yong,SHI Zhen-dan,CAO Yong-chang,BI Ying-zuo,MA Jing-yun. Cloning and sequencing analysis of myostatin gene of chicken and goose[J]. Journal of South China Agricultural University, 2004, 25(2): 85-88. DOI: 10.7671/j.issn.1001-411X.2004.02.022
    Citation: MA Xian-yong,SHI Zhen-dan,CAO Yong-chang,BI Ying-zuo,MA Jing-yun. Cloning and sequencing analysis of myostatin gene of chicken and goose[J]. Journal of South China Agricultural University, 2004, 25(2): 85-88. DOI: 10.7671/j.issn.1001-411X.2004.02.022
    shu

    鸡、鹅肌肉生成抑制因子基因的克隆及序列分析

    Cloning and sequencing analysis of myostatin gene of chicken and goose

      摘要
    • 摘要: 采用反转录．聚合酶链反应(RT-PCR)技术，从鸡胚、鹅胚中扩增得到肌肉生成抑制因子基因，分别克隆到pGEM-T Easy载体中进行序列测定．结果表明，克隆得到的MSTN基因序列均由1128个核苷酸组成，MSTN基因序列同源性为94．6％，推导相应氨基酸序列的同源性达97．9％．

       

      Abstract: The myostatin gene of chicken and goose was amplified by reverse transcription-polymerase chain reaction(RT-PCR),then cloned into pGEM-T Easy vector and sequenced separately. The result showed that the complete gene either obtained from chicken or goose skeletal muscle consisted of (1 128) nucleotides, chicken myostatin cDNA sequence shared 94.6% homology with goose myostatin cDNA sequence,and the homology of myostatin amino acids sequence between them was 97.9%, which was higher than that of the cDNA sequence.

       

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