Abstract: Using the method of matching design, the resistance to Plasmopara viticola and Sphaceloma ampelinum, as well as resistance relationship were studied with 8 varieties (strain) of Vitis vinifera and a hybrid of V. vinefera and V. amurensis by natural infection investigation in field and artificial inoculation evaluation on leaf disc in lab. The results showed that the distribution of the resistance to P. viticola was negative correlated with that of the resistance to S. ampelinum, but not significantly. So it could be inferrred that slightly effect multiple-genes which controlled P. viticola and S. ampelinum were not same gene-group, or were two gene-groups whose degree of chain were not strong. But the two diseases had some extent negative relationship indeed. That is to say, varieties having strong resistance to P. viticola had not same degree resistance to S. ampelinum, conversely, it was the same. The resistance of same variety to two disease emerged interlocking phenomena.