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    曹必好,雷建军,宋洪元,秦耀国. 芥菜农杆菌高效遗传转化体系初步建立[J]. 华南农业大学学报, 2003, 24(4): 48-51. DOI: 10.7671/j.issn.1001-411X.2003.04.013
    引用本文: 曹必好,雷建军,宋洪元,秦耀国. 芥菜农杆菌高效遗传转化体系初步建立[J]. 华南农业大学学报, 2003, 24(4): 48-51. DOI: 10.7671/j.issn.1001-411X.2003.04.013
    shu
    CAO Bi-hao~1,LEI Jian-jun~1,SONG Hong-yuan~2,QIN Yao-guo~1. Initial Establishment of Efficient and Stable Mustard Transgenic System Mediated with Agrobacterium tumefacciens[J]. Journal of South China Agricultural University, 2003, 24(4): 48-51. DOI: 10.7671/j.issn.1001-411X.2003.04.013
    Citation: CAO Bi-hao~1,LEI Jian-jun~1,SONG Hong-yuan~2,QIN Yao-guo~1. Initial Establishment of Efficient and Stable Mustard Transgenic System Mediated with Agrobacterium tumefacciens[J]. Journal of South China Agricultural University, 2003, 24(4): 48-51. DOI: 10.7671/j.issn.1001-411X.2003.04.013
    shu

    芥菜农杆菌高效遗传转化体系初步建立

    Initial Establishment of Efficient and Stable Mustard Transgenic System Mediated with Agrobacterium tumefacciens

      摘要
    • 摘要: 采用农杆菌导入法，探讨了影响芥菜农杆菌遗传转化效率的因素，优化了参数，初步建立了以MS 6-BA(2．0mg/L) NAA(0．2mg/L)为分化培养基，预培养时间3或4d，共培养时间4d，农杆菌浓度D600nm为O．5，浸菌时间10min，筛选压Kan为25mg/L的遗传转化体系。

       

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