Abstract: The genotype US66-56-9 of sugarcane leave was used as the material and to induce and to establish suspension cell culture system. Under the condition of phosphate sufficiency and deficiency, the comparison was made in terms of the changes of growth curves, cell water content rates and medium pH values of suspension cell, respectively. The results were followed: compared with the condition of phosphate sufficiency, phosphate deficiency inhibited cell growth, kept cell water content rate higher, maintained pH value of medium with acidic throughout cell growth.