Abstract: The effects of major nutrients in MS medium on suspension culture of sugarcane cell were studied, including different initial phosphate concentration, different nitrogen, total quantities of nitrogen, different sugar, different initial sucrose concentration. The results indicated that when the initial phosphate concentration was 1 25 mmol/L, the nitrogen was NH 4NO 3+KNO 3, the total quantities of nitrogen was 30 mmol/L, the sugar was sucrose and its initial concentration was 30 g/L, the growth of suspension culture of sugarcane cell was the best.