高级检索
    吴孔兴,黄青云,丘家军. 禽巴氏杆菌大肠杆菌融合二联弱毒菌株F4的培育[J]. 华南农业大学学报, 2002, 23(1): 67-70. DOI: 10.7671/j.issn.1001-411X.2002.01.020
    引用本文: 吴孔兴,黄青云,丘家军. 禽巴氏杆菌大肠杆菌融合二联弱毒菌株F4的培育[J]. 华南农业大学学报, 2002, 23(1): 67-70. DOI: 10.7671/j.issn.1001-411X.2002.01.020
    shu
    WU Kong xing 1,HUANG Qing yun 2,QIU Jia jun 3. Development of 2 - Combined Attenuated Strain F4 by Fusing Escherichia coli. O78 with Pasteurella multocida C48- 1[J]. Journal of South China Agricultural University, 2002, 23(1): 67-70. DOI: 10.7671/j.issn.1001-411X.2002.01.020
    Citation: WU Kong xing 1,HUANG Qing yun 2,QIU Jia jun 3. Development of 2 - Combined Attenuated Strain F4 by Fusing Escherichia coli. O78 with Pasteurella multocida C48- 1[J]. Journal of South China Agricultural University, 2002, 23(1): 67-70. DOI: 10.7671/j.issn.1001-411X.2002.01.020
    shu

    禽巴氏杆菌大肠杆菌融合二联弱毒菌株F4的培育

    Development of 2 - Combined Attenuated Strain F4 by Fusing Escherichia coli. O78 with Pasteurella multocida C48- 1

      摘要
    • 摘要: 以恩诺沙星标记禽多杀性巴氏杆菌强毒株C48－1得到的耐药菌株C48－1（Enr^r,Chl^8) ,与禽大肠杆菌O78弱毒株O78（Chl^r,Enr^8) 作为亲本菌株，采用溶菌酶－EDTA法制备两亲本菌株原生质体，通过聚乙二醇（PEG）－新生磷酸钙促融系统进行两亲本菌株原生质体融合，得到16株双耐药融合菌株。选取融合菌株F4、F9进行初步的免疫试验，初步显示融合菌株F4是一株安全、具双联免疫效果的融合菌株。

       

      Abstract: This paper deals mainly with the interfamily spheroplast fusion of Pasteurella multocida C 48-1 (Enr r,Chl s) and Escherichia coli O 78 (Chl r,Enr s), and the development of 2-combined attenuated strains. Protoplast fusion was performed by mixing protoplast suspension with 400 g/L PEG4 000 and newly formed Ca 3(PO 4) 2, and 16 strains hybrid cells with two drug-resistant markers were selected. To test the immunogenicity and safety of 2 fusion attenuated strains (F 4 and F 9), Guangdong yellow chicks were vaccinated separately with F 4 and F 9.The result showed that the F 4 strain was safe and with immunogenicity attributable to the two parent bacteria.

       

      • HTML全文
      • 参考文献(0)
      • 相关文章
      • 施引文献
    • 资源附件(0)

    /

    返回文章
    返回